China Animal Husbandry & Veterinary Medicine ›› 2011, Vol. 38 ›› Issue (9): 94-97.

• 生物技术 • Previous Articles     Next Articles

Preparation Monoclonal Antibodies of HN Protein Using Newcastle Disease Virus

YU Yang1,2, CUI Jia-ying2, DING Qing-wei2, CUI Peng-fei1, LI Yin2, DENG Guo-hua2, REN Tao1, CHEN Hua-lan2   

  1. 1. South China Agricultural University, Guangzhou 510642, China;2. Animal Influenza Laboratory of the Ministry of Agriculture/State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
  • Received:2011-01-17 Revised:1900-01-01 Online:2011-09-20 Published:2011-09-20

Abstract: In order to develop monoclonal antibodies of HN protein of Newcastle disease virus, BALB/c mice were immunized with CK/AH/100/2010 virus, and spleens were used for fusion with SP2/0. Six McAb cell strains of PX1 to PX6 were determined by hemagglutinin inhibition (HI). The results of HI cross-reaction with AIV, EDS and other HA virus suggested that the six McAbs had excellent specificity with NDV. The McAbs PX1, PX3 were shown to react with the HN antigen of NDV CK/GD/263/2010 in indirect-immunofluorescence assay.

Key words: Newcastle disease virus; monoclonal antibody; HN gene; HI

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