水貂肠炎病毒VP2基因在昆虫杆状病毒表达系统中的表达

›› 2011, Vol. 38 ›› Issue (9): 97-100.

• 生物技术 • Previous Articles Next Articles

Expression of VP2 Gene of Mink Enteritis Virus in Baculovrius Expression System

NI Jia, ZHANG Lei, CHAI Xiu-li, GAO Han, ZHANG Hai-ling, ZHAO Jian-jun, BAI Xue, SHAO Xi-qun, YAN Xi-jun

Laboratory of Preventive Veterinary Medicine, Institute of Special Wild Economic Animal and Plant Science, CAAS, Changchun 130122, China

Received:2011-01-25 Revised:1900-01-01 Online:2011-09-20 Published:2011-09-20

Abstract

Abstract: To obtain expression of VP2 gene of MEV in Bac-To-Bac expression system and develop MEV subunit vaccine. MEV VP2 gene was amplified by PCR method and then cloned into pMD18-T vetcor. The VP2 gene was inserted into pFast-BacⅠ vector,then transferred the recombinant plasmid pFast-BacⅠ-VP2 into DH10 Bac to get recombinant Bacmid. Subsequently,the recombinant bacmid was transfected into Sf9 cells. The expressed recombinant protein was identified by Western blotting after three cell passages.VP2 gene of MEV was successfully cloned. The obtained recombinant VP2 protein reacted with MEV monoclonal antibody was identified by Western blotting. The VP2 gene of MEV is successfully expressed in Bac-To-Bac expression system and shows well reactivity with MEV monoclonal antibody.

Key words: mink enteritis virus; VP2 gene; virus-like particles; baculovirus expression system

CLC Number:

S852.65⁺9.1

NI Jia;ZHANG Lei;CHAI Xiu-li;GAO Han;ZHANG Hai-ling;ZHAO Jian-jun;BAI Xue;SHAO Xi-qun;YAN Xi-jun. Expression of VP2 Gene of Mink Enteritis Virus in Baculovrius Expression System[J]. , 2011, 38(9): 97-100.

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Expression of VP2 Gene of Mink Enteritis Virus in Baculovrius Expression System

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