山羊痘病毒p32基因的克隆、表达及单克隆抗体制备

China Animal Husbandry & Veterinary Medicine ›› 2011, Vol. 38 ›› Issue (9): 101-105.

• 生物技术 • Previous Articles Next Articles

Clone,Expression and Monoclonal Antibody Preparation of p32 Gene of GPV

WANG Yong-zhi^1,3, SU Ying¹, MI Li-juan², FU Yun-hong², FAN Zhi-qiang³, HU Rong-liang³, ZHAO Yu-min¹

1. Jilin Academy of Agricultural Sciences, Changchun 130124, China;2. College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China;3. Veterinary Institute, Academy of Military Medical Sciences of PLA, Changchun 130062, China

Received:2011-01-21 Revised:1900-01-01 Online:2011-09-20 Published:2011-09-20

Abstract

Abstract: For expressing and preparing monoclonal antibody of CPV p32 protein,p32 gene was cloned and inserted into plasmid pET28a(+),and expressed efficiently in E.coli Rosseta by IPTG induction. After purification by affinity chromatograph,p32 protein was immunized as antigen to BALB/c mouse. 22 monoclonal antibodies which recognize recombinant p32 protein were obtained,among them,3 could recognize CPV. These monoclonal antibodies provided biomaterials for sandwich ELISA testing CPV and competitive ELISA testing CPV antibodies.

Key words: CPV; p32 gene; monoclonal antibody

CLC Number:

WANG Yong-zhi;SU Ying;MI Li-juan;FU Yun-hong;FAN Zhi-qiang;HU Rong-liang;ZHAO Yu-min. Clone,Expression and Monoclonal Antibody Preparation of p32 Gene of GPV[J]. China Animal Husbandry & Veterinary Medicine, 2011, 38(9): 101-105.

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Clone,Expression and Monoclonal Antibody Preparation of p32 Gene of GPV

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