应用实时荧光定量PCR测定牛体细胞端粒长度

›› 2010, Vol. 37 ›› Issue (9): 154-157.

• 遗传繁育 • Previous Articles Next Articles

Bovine Somatic Cell Telomere Length Measurement by Quantitative PCR

LV Kun，LIN Dan，XU Miao，ZHU Yi-wen，HUANG Ying

（Shanghai Institution of Medical Genetics, Shanghai Children’s Hospital，Shanghai Jiao Tong University School of Medicine，Key Laboratory of Embryo Molecular Biology，Ministry of Health; Shanghai Laboratory of Embryo and Reproduction Engineering，Shanghai 200040，China）

Received:1900-01-01 Revised:1900-01-01 Online:2010-09-20 Published:2010-09-20
Contact: HUANG Ying

Abstract

Abstract: In order to test the possibility of quantitative PCR (Q-PCR) in the measurement of bovine telomere length, we chose 18 different DNA samples of bovine ear fibroblast, and then analyzed the relativity of quantitative PCR and Southern blotting methods. The results showed that the relative T/S ratio of Q-PCR was 1.16±0.24, the mean TRF of Southern blotting was 16.99 kb±0.85 kb.The analysis of the relativity of two methods showed that R2= 0.5612(P<0.01), so we got the conclusion that Q-PCR was a reliable method in the measurement of bovine telomere length.

Key words: quantitative PCR; Southern blotting; bovine somatic cell; telomere length

CLC Number:

S814.8

LV Kun;LIN Dan;XU Miao;ZHU Yi-wen;HUANG Ying. Bovine Somatic Cell Telomere Length Measurement by Quantitative PCR[J]. , 2010, 37(9): 154-157.

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Bovine Somatic Cell Telomere Length Measurement by Quantitative PCR

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