Abstract: The sperm carrier method is to use sperm as carrier to ducting exogenous gene when the conceive process in,making exogenous gene get into the gene set of son generation thus.This text synopsis introduct the mechanism of the sperm combine of exogenous gene,the method that sperm and exogenous gene combine, the influencing factor of sperm transfecting exogenous gene，and producing sperm of the transgenic animal.This text synopsis also give an overview of transfecting method of injection vas deferens, the method of microinjection vas contorted seminiferous tubules,the method of testicle direct injection and other new path of the sperm carrier method.

Key words: sperm carrier method; transgene; exogenous DNA