雪白睡莲花粗多糖提取工艺优化及其对金黄色葡萄球菌性小鼠乳腺炎的治疗效果研究

doi:10.16431/j.cnki.1671-7236.2025.06.034

Abstract

Abstract: 【Objective】 The aim of the experiment was to optimize the extraction process of crude polysaccharide from Nymphaea candida and explore its application effect in the treatment of mastitis in mice infected with Staphylococcus aureus.【Method】 The experiment used Nymphaea candida as the test material,and the extraction rate of Nymphaea candida crude polysaccharide as the evaluation index,and the one-way factor combined with response surface method was used to optimize its extraction process.At the same time,60 SPF healthy Kunming mice were randomly divided into four groups:Blank control group (BC),model control group (MC),positive control group (PC),and crude polysaccharides from Nymphaea candida (CPNA) group,15 mice in each group.50 μL Staphylococcus aureus with 1×10⁴ CFU/mL was injected into mice in each group except for blank control group to construct a mouse mastitis model.After successful modelling,saline,0.13 g/kg ciprofloxacin,and 0.5 g/kg crude polysaccharides from Nymphaea candida were administered by gavage to mice in each group twice a day for 7 d,respectively.Mice in blank control group were not treated.The number of Staphylococcus aureus colonies,the content of tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in mammary tissues of mice in each test group were measured at 3,5 and 7 d of administration,respectively,and the pathological histological changes in mammary glands of the mice were also observed to evaluate the therapeutic effect.【Result】 Under the conditions of material-liquid ratio was 1∶14,ultrasonic power was 70%,extraction temperature was 57 ℃,extraction time was 30 min,and the number of extraction times was 2,the yield of Nymphaea candida crude polysaccharide was 51.78%.On the 5th and 7th days,the number of colonies in mammary tissue,TNF-α and IL-6 contents of mice in CPNA group showed significantly or extremely significantly lower than that in model control group (P＜0.05 or P＜0.01),and the inflammatory cells and erythrocytes in mammary tissues of mice were significantly reduced.【Conclusion】 After optimizing the extraction method using one-factor combined response surface method,the yield of crude polysaccharide from Nymphaea candida was 51.78%.It had a good therapeutic effect on the animal model of mouse mastitis induced by Staphylococcus aureus.The results of this study could provide a scientific basis for the prevention and treatment of clinical mastitis and the development and utilization of Nymphaea candida.

Key words: Nymphaea candida; crude polysaccharide; extraction process; Staphylococcus aureus; mastitis

CLC Number:

S853.7

LIU Dandan, LUO jie, GAO Hanya, XU Liyina, HAO Baoshan, WANG Xin, ZHANG Wei. Optimization of the Extraction Process of Crude Polysaccharides from Nymphaea candida and Its Therapeutic Effect on Mouse Mastitis Induced by Staphylococcus aureus[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(6): 2818-2829.

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Optimization of the Extraction Process of Crude Polysaccharides from Nymphaea candida and Its Therapeutic Effect on Mouse Mastitis Induced by Staphylococcus aureus

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