基于不同ST型肺炎克雷伯菌感染的小鼠乳腺炎模型的差异分析

doi:10.16431/j.cnki.1671-7236.2025.01.038

Abstract

Abstract: 【Objective】 Klebsiella pneumoniae infection is a common cause of mastitis in dairy cows,which induces acute clinical mastitis with severe clinical symptoms,resulting in reduced milk production and quality,thereby causing significant economic losses to dairy farms.The purpose of this study was to explore the virulence effects of different sequence types (ST) of Klebsiella pneumoniae originating from cow mastitis,and screen for potential key virulence genes that contribute to the infectious role of Klebsiella pneumoniae. 【Method】 The multi-locus sequence typing (MLST) method was employed to analyze the distribution of ST among 129 strains of Klebsiella pneumoniae isolated from clinical mastitis cases in two large dairy farms located in Shandong and Hubei provinces.Subsequently,3 of the most prevalent ST of Klebsiella pneumoniae were selected to establish mastitis model in mice,and the morphological damage and expression changes of inflammatory factors in mammary glands of mice were investigated.The key virulence genes associated with Klebsiella pneumoniae infection were screened by analyzing the infection characteristics and virulence gene distribution of Klebsiella pneumoniae. 【Result】 129 strains of Klebsiella pneumoniae were categorized into 79 ST types,of which ST107 accounted for the highest proportion of 19.37%,followed by ST2324 (7.75%) and ST13 (3.87%).Compared with ST107 and ST13,ST2324 induced Klebsiella pneumoniae clinical score and mammary gland bacteria load were significantly increased 24 h after infection (P ＜ 0.05).The acinar contour disappeared and a large number of inflammatory cells infiltrated,and the mRNA expressions of interleukin-1β (IL-1β),tumor necrosis factor-α (TNF-α),and cytochrome C (Cyt C) were significantly increased (P ＜ 0.05).Virulence gene detection results showed that 74 virulence genes were detected in 3 different ST isolates,among which the virulence genes manB, pla and pilW were detected in ST2324 but not in ST13 and ST107. 【Conclusion】 In this study,a total of 79 ST types were detected from 129 strains of Klebsiella pneumoniae,among which ST2324,ST107 and ST13 were detected with high rates,and ST2324 had the strongest pathogenicity to mice,and the virulence genes manB,pla and pilW were closely related to the high pathogenicity of Klebsiella pneumoniae.The results of this study provided a theoretical reference for further analysis of the mechanism of Klebsiella pneumoniae infection of dairy cow mastitis.

Key words: dairy cow mastitis; Klebsiella pneumoniae; sequence type (ST); virulence gene

CLC Number:

S858.292

REN Meiyi, LIANG Hongxiu, LI Can, FENG Mingque, XIE Qinna, SONG Deyuan, LIU Mingchao, GAO Jian, CHENG Jia. Differential Analysis of Mastitis Model in Mice Infected with Klebsiella pneumoniae of Different ST Types[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(1): 422-431.

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Differential Analysis of Mastitis Model in Mice Infected with Klebsiella pneumoniae of Different ST Types

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