新疆某规模化奶牛场乳源金黄色葡萄球菌的分离鉴定、耐药分析及毒力基因检测

doi:10.16431/j.cnki.1671-7236.2025.03.038

Abstract

Abstract: 【Objective】 The purpose of this experiment was to explore the drug resistance and virulence gene carrying of Staphylococcus aureus (S.aureus)in a large-scale dairy farm in Xinjiang,so as to provide a certain reference for the prevention and control of S.aureus in this farm.【Method】 In this study,milk samples were collected from the large-scale dairy farm,the bacteria were isolated and purified by plate scribing method,the strains were identified by S.aureus-specific primers,the antibiotic resistance phenotype of the isolate was analyzed by Kirby-Bauer method,and the antibiotic resistance and virulence genes were detected by PCR method.【Result】 The average isolation rate of S.aureus in 262 milk samples was 15.27% (40/262),of which 28.57% (10/35) in clinical mastitis milk samples,and 11.41% (17/149) and 16.67% (13/78) in recessive mastitis milk samples and healthy milk samples,respectively.5.00% (2/40) of the isolates were not detected with housekeeping genes (arcC,aroE,glpF,gmK,pta,tpi and yqil genes).The detection rate of virulence genes clfA,clfB and coa was high.There were 16 different virulence gene combinations.77.50% of the isolates were multidrug-resistant strains,and one strain was resistant to 14 drugs including vancomycin.The detection rates of lincoamide resistance gene linA (52.50%),quinolone resistance gene qnrA (50.00%),aminoglycoside resistance gene aacA-aphD (45.00%),erythromycin and clindamycin resistance gene ermC (40.00%) were higher in isolates.The antibiotic resistance phenotypes of five kinds of antibiotics such as chloramphenicol had a high degree of overlap with antibiotic resistance genes,while the antibiotic resistance phenotypes of three kinds of drugs,including β-lactams,had a low degree of overlap with the corresponding resistance genes.【Conclusion】The results indicated that the milk-derived S.aureus from the farm was generally antibiotic resistant,and the phenomenon of multiple antibiotic resistance was more serious.The results of this study provided a reference for the prevention and control of S.aureus in the farm.

Key words: dairy cow; Staphylococcus aureus; drug resistance; virulence gene

CLC Number:

S852.61

LI Yang, XU Jingjing, ZHANG Xiaoyu, LI Nana, YU Xingyu, LENG Qingwen, LI Yanfang, QU Yonggang. Isolation,Identification,Drug Resistance Analysis,and Virulence Gene Detection of Staphylococcus aureus from a Large-scale Dairy Farm in Xinjiang[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(3): 1370-1382.

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Isolation,Identification,Drug Resistance Analysis,and Virulence Gene Detection of Staphylococcus aureus from a Large-scale Dairy Farm in Xinjiang

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