1株贝莱斯芽孢杆菌G02全基因组测序分析

doi:10.16431/j.cnki.1671-7236.2025.05.004

Abstract

Abstract: 【Objective】 The whole genome sequence analysis of Bacillus velezensis G02 was carried out to explore the genes related to its antibacterial and bacteriogenic properties in order to analyze its genome information in detail.【Method】 The whole gene of Bacillus velezensis G02 was sequenced by Illumina NovaSeq 6000 platform using next-generation sequencing technology.Genome characteristics were analyzed by gene assembly,genome length,functional annotation and secondary metabolite prediction.【Result】 The whole genome sequencing results showed that the total length of Bacillus velezensis G02 genome was 3 891 653 bp,with 3 749 protein-coding genes and a GC content of 46%.The genome sequence consists of 12 contigs with 92 non-coding RNA,including 8 5S rRNA,2 16S rRNA,1 23S rRNA,80 trRNA and 1 tmRNA.Functional annotation results showed that 9 genes related to antioxidant activity were annotated in GO database.KEGG database annotated 31 pathways,among wihch the genes involved in bacterin synthesis and immune-related pathways were ko00253,ko00261 and ko00521,etc.A total of 12 secondary metabolite synthesis gene clusters were annotated by antiSMASH online prediction software.【Conclusion】 Bacillus velezensis G02 was a potential strain producing antibacterial active substances,and the results provided a basis for the development and exploration of the antibacterial mechanism of Bacillus velezensis G02.

Key words: Bacillus; whole genome sequencing; secondary metabolites; function annotation

CLC Number:

S852.61⁺6

YU Mengbo, MA Haotian, LI Yang, PAN Ruixue, LONG Yuner, ZHAO Yining, MA Yi, ZHOU Shuzhen. Whole Genome Sequencing Analysis of a Strain of Bacillus velezensis G02[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(5): 1977-1986.

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Whole Genome Sequencing Analysis of a Strain of Bacillus velezensis G02

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