1株鸡源奇异变形杆菌的全基因组测序及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2025.03.001

Abstract

Abstract: 【Objective】 Proteus mirabilis (P. mirabilis),a prevalent zoonotic pathogen,was commonly found in the natural environment.The escalating resistance of P. mirabilis posed a significant threat to public health and safety,prompting widespread concern.This study was aimed to analyze the genomic data,virulence factors,and drug resistance gene profiles of P. mirabilis NXQI.22 isolated from chickens,and provide a bioinformatic basis for further investigation of the mechanism of action of the drug against multidrug-resistant P.mirabilis.【Method】 Employing the third-generation Nanopore platform,whole genome sequencing and bioinformatics analysis were conducted on P. mirabilis NXQI.22 to elucidate its genomic features and drug resistance mechanisms.【Result】 The genomic analysis revealed that P. mirabilis NXQI.22 harbored a circular chromosome measuring 3 862 364 bp with a GC content of 38.83%.A total of 3 551 coding genes were predicted in P.mirabilis NXQI.22.Six prophages and two CRISPR sequences were identified in P. mirabilis NXQI.22,along with the presence of typical T3SS and T6SS secretion system proteins.This strain also harbored 15 gene islands,genes conferring resistance to sulfonamides and disinfectants,as well as various virulence genes associated with the secretion system and effector proteins found in Salmonella.The annotation results showed that the isolated strains harbored a total of 362 virulence genes associated with functions such as iron uptake,cellular adhesion,proteolysis,resistance to antibiotics,and the formation of biofilms.It contained a diverse array of genes associated with resistance to various drugs,including quinolones,aminoglycosides,tetracyclines,β-lactams,and quaternary ammonium compound.Resistance mechanisms employed by these strains encompassed antibiotic efflux,antibiotic inactivation,antibiotic target alteration and replacement,and multiple drug resistance genes showed multiple drug resistance phenotypes.Multiple virulence genes and drug resistance genes in P. mirabilis NXQI.22 were associated with mobile genetic elements.【Conclusion】 P. mirabilis NXQI.22 from chicken harbored a significant number of virulence genes and drug resistance genes.

Key words: Proteus mirabilis; whole genome sequencing; virulence genes; drug resistance genes

CLC Number:

S852.61

RU Mengke, LI Suixiang, WU Xueqin, YAN Yuzhang, WANG Lu, CHENG Haipeng. Whole Genome Sequencing and Bioinformatics Analysis of a Strain of Proteus mirabilis Isolated from Chicken[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(3): 977-989.

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Whole Genome Sequencing and Bioinformatics Analysis of a Strain of Proteus mirabilis Isolated from Chicken

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