畜禽粪污堆肥病原体及抗生素抗性基因去除效果研究进展

doi:10.16431/j.cnki.1671-7236.2024.10.045

Abstract

Abstract: Livestock manure contains a large number of pathogenic microorganisms，including pathogenic bacteria and viruses，along with detrimental components such as antibiotics and antibiotic resistance genes (ARGs)，thereby presenting a grave threat to the ecological environment and the health of both humans and animals. Therefore，the harmless treatment of these livestock manure is an important prerequisite for realizing the resource utilization of these organic fertilizer raw materials. Aerobic composting has been widely proved to be one of the most effective ways to realize the harmless and resource recycling of livestock manure. By reviewing the relevant literature on the removal efficiency of pathogens，antibiotics and ARGs in livestock manure compost at home and abroad，the author provides a brief description of the harmfulness of pathogens，antibiotics and ARGs，and conducts a cursory analysis of the fundamental principle for eliminating these harmful components during the composting process. Additionally，the related research reports on the impact of temperature，pH，carbon-to-nitrogen ratio，moisture content and additives on the elimination of these harmful components in compost were reviewed in detail，and the reasons and effects of these factors on the removal of pathogens，antibiotics and ARGs were discussed. It was discovered that the present research on viruses in livestock manure compost is highly inadequate, and suggests that in the future, metagenomics technology and macro virion technology can be combined to analyze the abundance and community structure of viruses during livestock and poultry manure composting, and further analyze the functions of viruses. In addition, the research on the removal of antibiotics and ARGs is not all-round, and the biosafety research on some new ways of composting is still lacking, so more research efforts are needed.This paper can provide the basis for the safe production of livestock and poultry manure compost and provide the direction for future research.

Key words: livestock and poultry manure; pathogens; antibiotic resistance gene; removal effect

CLC Number:

S811
S141.4

LI Rongrong, CAI Rui. Research Progress on the Removal Effect of Pathogens and Antibiotic Resistance Genes in Manure Compost of Livestock and Poultry[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(10): 4643-4652.

References

[1] BLOME S，FRANZKE K，BEER M. African swine fever：A review of current knowledge[J]. Virus Research，2020，287：198099.
[2] BI Y，LI J，LI S Q，et al. Dominant subtype switch in Avian influenza viruses during 2016-2019 in China[J]. Nature Communications，2020,11(1):1-12.
[3] 李明章,李彦明,王珏,等.好氧堆肥去除畜禽粪便病原体的研究进展[J].农业资源与环境学报,2023,40(4):864-872.LI M Z，LI Y M，WANG J，et al. Research progress on aerobic composting for removing pathogens from livestock manure[J]. Journal of Agricultural Resources and Environment，2023，40(4)： 864-872. (in Chinese)
[4] MASSE D，GILBERT Y，TOPP E. Pathogen removal in farm-scale psychrophilic anaerobic digesters processing swine manure[J]. Bioresource Technology，2011,102(2)：641-646.
[5] CHRIST M L，SAMPSON M，EDSON M，et al. An overview of the control of bacterial pathogens in cattle manure[J]. International Journal of Environmental Research and Public Health，2016，13(9)： 843.
[6] 王为，王蓓蕾，胡桂学，等. 空肠弯曲菌在环境压力条件下的生存机制和可培养性研究进展[J].肉类研究,2011,25(5)：37-39.WANG W，WANG B L，HU G X，et al. Recent advances in research on survival mechanisms and culturability of Campylobacter jejuni under stress conditions[J]. Meat Research,2011,25(5)：37-39. (in Chinese)
[7] GALE P. Risks to farm animals from pathogens in composted catering waste containing meat[J]. Veterinary Record，2004，155(3)： 77-82.
[8] ELVING J，EMMOTH E，ALBIHN A，et al. Composting for Avian influenza virus elimination [J]. Applied and Environmental Microbiology，2012，78(9)：3280-3285.
[9] 中国疾病预防控制中心环境与健康相关产品安全所.粪便无害化卫生要求：GB 7959—2012[S].北京：中国标准出版社,2012.CHINA CENTER FOR DISEASE CONTROL AND PREVENTION ENVIRONMENTAL HEALTH RELATED PRODUCT SAFETY INSTITUTE. Hygienic requirements of fecal safety：GB 7959—2012[S]. Beijing： Standards Press of China，2012. (in Chinese)
[10] SAVEYN H，EDER P. End-of-waste Criteria for Biodegradable Waste Subjected to Biological Treatment(Compost and Digest): Technical Proposals[M]. Seville： Publications Office of the European Union，2014.
[11] 钟为章，陈赛男，李月，等. 好氧堆肥对抗生素抗性基因的消长影响研究进展[J].应用化工,2022,51(7)：2057-2063.ZHONG W Z，CHEN S N，LI Y，et al. Research progress on the effect of aerobic composting on the growth and decline of antibiotic resistance genes[J]. Applied Chemical Industry,2022,51(7)：2057-2063. (in Chinese)
[12] WANG M M，CAI C，ZHANG B，et al. Characterization and mechanism analysis of lincomycin biodegradation with Clostridium sp. strain LCM-B isolated from lincomycin mycelial residue (LMR)[J].Chemosphere,2018,193：611-617.
[13] 朱为静，朱凤香，王卫平，等. 4种粪便堆肥过程中抗生素的降解特性[J].环境科学,2020,41(2):1005-1012.ZHU W J，ZHU F X，WANG W P，et al. Degradation characteristics of antibiotics during composting of four types of feces[J]. Environmental Science，2020,41(2)：1005-1012. (in Chinese)
[14] BOUND J P，VOULVOULIS N. Pharmaceuticals in the aquatic environment-A comparison of risk assessment strategies[J].Chemosphere,2004,56(11)：1143-1155.
[15] 郭丽，王淑平. 兽用抗生素在土壤中运移规律研究进展[J].环境科学与技术,2014,37(120)：237-246.GUO L，WANG S P. Migration law of veterinary antibiotics in soil[J]. Environmental Science & Technology，2014，37(12)：237-246.(in Chinese)
[16] GUAN T Y，HOLLEY R A. Pathogen survival in swine manure environments and transmission of human enteric illness——A review[J].Journal of Environmental Quality,2003,32(2)：383-392.
[17] 刘昌孝. 全球关注:重视抗生素发展与耐药风险的对策[J].中国抗生素杂志,2019,44(1)：1-8.LIU C X. Global concern: Strategies for antibiotic development and risk of resistance[J]. Chinese Journal of Antibiotics,2019,44(1)：1-8. (in Chinese)
[18] 李国学，张福锁. 固体废物堆肥化与有机复混肥生产[M]. 北京：化学工业出版社,2000.LI G X，ZHANG F S. Solid Waste Composting and Organic Compound Fertilizer Production[M]. Beijing： Chemical Industry Press,2000.(in Chinese)
[19] HIMATHONGKHAM S，BAHARI S，RIEMANN H，et al. Survival of Escherichia coli O157∶H7 and Salmonella Typhimurium in cow manure and cow manureslurry[J]. FEMS Microbiology Letters,1999,178(2)：251-257.
[20] ZALESKI K J，JOSEPHSON K L，GERBA C P，et al. Survival，growth，and regrowth of enteric indicator and pathogenic bacteria in biosolids，compost，soil，and land applied biosolids[J]. Journal of Residualsence ＆ Technology,2005,2(1)：49-63.
[21] CHEN Z，KIM J，JIANG X. Survival of Escherichia coli O157∶H7 and Salmonella enterica in animal waste-based composts as influenced by compost type，storage condition and inoculum level[J]. Journal of Applied Microbiology，2018，124(5)：1311-1323.
[22] GREWAL S，SREEVATSAN S，MICHEL JR F C. Persistence of Listeria and Salmonella during swine manure treatment[J]. Compostence and Utilization，2007,15(1)：53-62.
[23] HO Y B，ZAKARIA M P，LATIF P A，et al. Degradation of veterinary antibiotics and hormone during broiler manure composting[J]. Bioresource Technology，2013,131：476-484.
[24] MILLER J H，NOVAK J T，KNOCKE W R，et al. Survival of antibiotic resistant bacteria and horizontal gene transfer control antibiotic resistance gene content in anaerobic digesters[J]. Frontiers in Microbiology，2016，7：263.
[25] 李纤慧，李建政，张成成，等. 畜禽粪便中抗生素抗性基因的分布特征及消减技术研究进展[J].微生物学报,2022,62(12)：4740-4755.LI X H，LI J Z，ZHANG C C,et al. Distribution characteristics of antibiotic resistance genes in livestock manure and the reduction techniques: A review[J]. Acta Microbiologica Sinica，2022，62(12)：4740-4755. (in Chinese)
[26] GUAN J，WASTY A，GRENIER C，et al. Influence of temperature on survival and conjugative transfer of multiple antibiotic-resistant plasmids in chicken manure and compost microcosms[J]. Poultry Science,2007，86(4)：610-613.
[27] PENG S，LI H J，SONG D，et al. Influence of zeolite and superphosphate as additives on antibiotic resistance genes and bacterial communities during factory-scale chicken manure composting[J]. Bioresource Technology,2018,263：393-401.
[28] LIANG J D，JIN Y M，WEN X，et al. Adding a complex microbial agent twice to the composting of layinghen manure promoted doxycycline degradation with a low risk on spreading tetracycline resistance genes[J]. Environmental Pollution,2020,265：114202.
[29] GUO H H，GU J，WANG X J，et al. Responses of antibiotic and heavy metal resistance genes to bamboo charcoal and bamboo vinegar during aerobic composting[J]. Environmental Pollution,2019,252：1097-1105.
[30] QIAN X，GU J，SUN W，et al. Effects of passivators on antibiotic resistance genes and related mechanisms during composting of copper-enriched pig manure[J]. Science of the Total Environment,2019,674：383-391.
[31] LIAO H P，ZHAO Q，CUI P，et al. Efficient reduction of antibiotic residues and associated resistance genes in tylosin antibiotic fermentation waste using hyperthermophilic composting[J]. Environment International,2019,113：105203.
[32] ARIKAN O A，MULBR Y W，RICE C. Management of antibiotic residues from agricultural sources: Use of composting to reduce chlortetracycline residues in beef manure from treated animals[J]. Journal of Hazardous Materials,2009,164(2-3)：483-489.
[33] WU N，XIE S Y，ZENG M，et al. Impacts of pile temperature on antibiotic resistance，metal resistance and microbial community during swine manure composting[J].Science of the Total Environment,2020,744：140920.
[34] CHEN Z Q，FU Q Q，WEN Q X，et al. Microbial community competition rather than high-temperature predominates ARGs elimination in swine manure composting[J]. Journal of Hazardous Materials，2022，423：127149.
[35] INOUE K，INANAGA S，SOMEYA T. Survival of pathogenic bacteria in compost with special reference to Escherichia coli[J]. Journal of Environmental Sciences,2015,17(5)：770-774.
[36] 周亚文，张宇航，沈玉君，等. 初始含水率对人粪污好氧堆肥腐熟及微生物群落结构变化的影响[J].环境工程学报,2022,16(12)：4108-4120.ZHOU Y W，ZHANG Y H，SHEN Y J，et al. Effect of the initial moisture content on the maturity extent and the microbial community structure of the aerobic compost with human excrement[J]. Chinese Journal of Environmental Engineering，2022，16(12)：4108-4120. (in Chinese)
[37] WANG Q Q，YATES S R. Laboratory study of oxytetracycline degradation kinetics in animal manure and soil[J]. Journal of Agricultural and Food Chemistry,2008，56(5)：1683-1688.
[38] 沈颖，魏源送，郑嘉熹，等. 猪粪中四环素类抗生素残留物的生物降解[J].过程工程学报,2009,9(5)：962-968.SHEN Y，WEI Y S，ZHENG J X，et al. Biodegradation of tetracycline antibiotic residues in pig manure[J]. The Chinese Journal of Process Engineering,2009,9(5)：962-968. (in Chinese)
[39] LIU B T，YU K F，AHMED I，et al. Key factors driving the fate of antibiotic resistance genes and controlling strategies during aerobic composting of animal manure: A review[J].Science of the Total Environment,2021,791：148372.
[40] CHEUNG H，HUANG G H，YU H. Microbial-growth inhibition during composting of food waste: Effects of organic acids[J]. Bioresource Technology，2010，101(15)：5925-5934.
[41] 李吉进. 畜禽粪便高温堆肥机理与应用研究[D].北京：中国农业大学，2004.LI J J. Research on mechanism and application of high temperature composting of livestock and poultry manure [D]. Beijing: China Agricultural University，2004. (in Chinese)
[42] LEPESTEUR M. Human and livestock pathogens and their control during composting[J]. Critical Reviews in Environmental Science and Technology，2021,52(10)：1639-1683.
[43] HUANG H N，CHEN Y G，ZHENG X，et al. Distribution of tetracycline resistance genes in anaerobic treatment of waste sludge: The role of pH in regulating tetracycline resistant bacteria and horizontal gene transfer[J]. Bioresource Technology,2016,218：1284-1289.
[44] DENG W W，ZHANG A Y，CHEN S J，et al. Heavy metals，antibiotics and nutrients affect the bacterial community and resistance genes in chicken manure composting and fertilized soil[J]. Journal of Environmental Management,2020,257：109980.
[45] 蔡瑞,徐春城.堆肥用微生物及其效果研究进展[J].中国土壤与肥料,2019，5:1-7.CAI R，XU C C. Research progress on microorganisms commonly used in composting and their effects[J].Soil and Fertilizer Sciences in China,2019，5:1-7.(in Chinese)
[46] LI H Y，CHENG W M，LI B H，et al. The fate of antibiotic resistance genes during co-composting of swine manure with cauliflower and corn straw[J]. Bioresource Technology,2020,300：122669.
[47] ERICKSON M C，LIAO J，MA L，et al. Inactivation of Salmonella spp. in cow manure composts formulated to different initial C:N ratios[J]. Bioresource Technology,2009,100(23)：5898-5903.
[48] LAFOND S，PARE'T，DINEL H，et al. Composting duck excreta enriched wood shavings: C and N transformations and bacterial pathogen reductions[J]. Journal of Environmental Science and Health (Part B)，2002，37(2)：173-186.
[49] 潘兰佳，唐晓达，汪印. 畜禽粪便堆肥降解残留抗生素的研究进展[J].环境科学与技术,2015,38(S2)：191-198.PAN L J，TANG X D，WANG Y. Research progress of residual antibiotics degradation in livestock and poultry feces composting[J]. Environmental Science & Technology，2015，38(12)：191-198. (in Chinese)
[50] ZHU P P，QIN H Y，ZHANG H，et al. Variations in antibiotic resistance genes and removal mechanisms induced by C/N ratio of substrate during composting[J]. Science of the Total Environment,2021,798：149288.
[51] SAVAGE J，CHASE T，MACMILLAN J D. Population changes in enteric bacteria and other microorganisms during aerobic thermophilic windrow composting[J]. Applied Microbiology，1973，26：969-974.
[52] MANGA M，MUOGHALU C，CAMARGO-VALERO M A，et al. Effect of turning frequency on the survival of fecal indicator microorganisms during aerobic composting of fecal sludge with sawdust[J]. International Journal of Environmental Research and Public Health,2023,20(3)：2668.
[53] KONÉ D，COFIE O，ZURBRVGG C，et al. Helminth eggs inactivation efficiency by faecal sludge dewatering and co-composting in tropical climates[J]. Water Research，2007，41(19)：4397-4402.
[54] FUHRIMANN S，STALDER M，WINKLER M S，et al. Microbial and chemical contamination of water，sediment and soil in the Nakivubo wetland area in Kampala，Uganda[J].Environmental Monitoring and Assessment,2017,187：475.
[55] 蔡瑞.青藏高原羊粪堆肥养分转化机理及生物安全研究[D].北京：中国农业大学，2023.CAI R. Mechanism nutrient of conversion and biosafety of sheep manure composting on the Qinghai-Tibet Plateau[D].Beijing：China Agricultural University,2023. (in Chinese)
[56] CHUNG W J，CHANG S W，CHAUDHARY D K，et al. Effect of biochar amendment on compost quality，gaseous emissions and pathogen reduction during in-vessel composting of chicken manure[J]. Chemosphere,2021,283：131129.
[57] 李春艳，刘立英，丁国志，等. 生物炭和微生物菌剂联用对鸡粪堆肥有害生物的影响[J].当代畜禽养殖业,2022,3：12-15.LI C Y，LIU L Y，DING G Z，et al. Effects of combination of biochar and microbial agent on pests in chicken manure compost[J]. Contemporary Livestock and Poultry Industry，2022，3：12-15. (in Chinese)
[58] 张天华. 牛粪强制通风静态堆肥中添加石灰氮对大肠杆菌的杀灭效果及相关指标的测定[D].呼和浩特：内蒙古农业大学,2012.ZHANG T H. The effect of adding lime nitrogen to cattle manure forced aerated static composting on killing Escherichia coli and the determination of related indicators [D]. Hohhot：Inner Mongolia Agricultural University，2012. (in Chinese)
[59] 李玉. 牛粪堆肥中添加石灰氮对粪肠球菌的杀灭效果及堆肥发酵影响的研究[D].呼和浩特：内蒙古农业大学,2011.LI Y. The effect of adding lime nitrogen to cattle manure compost on the killing effect of Enterococcus faecalis and composting[D]. Hohhot：Inner Mongolia Agricultural University，2011. (in Chinese)
[60] 白同日格. 牛粪堆肥中添加石灰氮对金黄色葡萄球菌的杀灭效果及堆肥发酵影响的研究[D].呼和浩特：内蒙古农业大学,2011.BAI T R G. The effect of adding lime nitrogen to cattle manure composting on killing impact of S. aureus and composting[D]. Hohhot: Inner Mongolia Agricultural University，2011. (in Chinese)
[61] WONG J W C，SELVAM A. Reduction of indicator and pathogenic microorganisms in pig manure through fly ash and lime addition during alkaline stabilization[J]. Journal of Hazardous Materials，2009，169(1-3)：882-889.
[62] 邵栓，朱群，李慧娟，等. 复合微生物菌剂在猪粪无害化处理中的应用[J].畜牧与兽医,2021,53(4)：42-47.SHAO S，ZHU Q，LI H J，et al. Application of compound microbial preparation in innocuous treatment of pig manure [J]. Animal Husbandry ＆ Veterinary Medicine,2021,53(4)：42-47.(in Chinese)
[63] 胡菊. VT菌剂在好氧堆肥中的作用机理及肥效研究[D].北京：中国农业大学,2005.HU J. The mechanism of aerobic composting with the inoculation of VT agent and the filed testing of composting products[D].Beijing：China Agricultural University,2005. (in Chinese)
[64] 陈宏宇. 粘土作为添加对鸡粪好氧堆肥的影响[D].杨凌：西北农林科技大学,2019.CHEN H Y. Effect of clay as additive to the chicken manure composting[D]. Yangling：Northwest A & F University，2019. (in Chinese)
[65] MONROY F，AIRA M，DOMÍNGUEZ J. Reduction of total coliform numbers during vermicomposting is caused by short-term direct effects of earthworms on microorganisms and depends on the dose of application of pig slurry[J]. Science of the Total Environment，2009,407：5411-5416.
[66] TONG Z Y，LIU F W，TIAN Y，et al. Effect of biochar on antibiotics and antibiotic resistance genes variations during co-composting of pig manure and corn straw[J]. Frontiers in Bioengineering and Biotechnology,2022,10：960476.
[67] 包灿鑫，张园，陈明龙，等. 不同生物炭对猪粪堆肥过程中抗生素及抗生素抗性基因的削减规律研究[J].环境污染与防治,2022,44(2)：160-164.BAO C X，ZHANG Y，CHEN M L，et al. Study on reducing rule of different biochar on antibiotics and ARGs in the composting process of pig manure [J].Environmental Pollution and Control,2022,44(2)：160-164.(in Chinese)
[68] 沈东升，何虹蓁，汪美贞，等. 土霉素降解菌TJ-1在猪粪无害化处理中的作用[J].环境科学学报,2013,33(1)：147-153.SHEN D S，HE H Z，WANG M Z，et al. The role of oxytetracycline-degrading bacterium TJ-1 on the hazard-free treatment of pig manure[J]. Acta Scientiae Circumstantiae，2013，33(1)：147-153. (in Chinese)
[69] HAO X X，ZHANG W J，ZHAO L B，et al. Bacillus subtilis reduces antibiotic resistance genes of animal sludge in vermicomposting by improving heat stress tolerance of Eisenia foetida and bacterial community adjustment[J]. Environmental Research,2023,219：115088.
[70] 孟磊，杨兵，薛南冬，等. 高温堆肥对鸡粪中氟喹诺酮类抗生素的去除[J].农业环境科学学报,2015,34(2)：377-383.MENG L，YANG B，XUE N D，et al. Effect of high temperature composting on removal of fluoroquinolones in chicken manures[J]. Journal of Agro-Environment Science,2015,34(2)：377-383. (in Chinese)
[71] QIU X W，FENG M T，ZHOU G X，et al. Effects of mineral additives on antibiotic resistance genes and related mechanisms during chicken manure composting[J]. Bioresource Technology,2021,346：126631.
[72] LI Y X，GU J，WANG X J，et al. The fate of antibiotic resistance genes and their influential factors in swine manure composting with sepiolite as additive[J]. Bioresource Technology,2022,347：126727.
[73] WEI Y，GU J，WANG X J，et al. Elucidating the beneficial effects of diatomite for reducing abundances of antibiotic resistance genes during swine manure composting[J].Science of the Total Environment,2022,821：153199.
[74] 陈景阳，夏慧，黄魁，等. 四环素对污泥蚯蚓粪中微生物种群和抗性基因的影响[J].环境科学,2019,40(7)：3263-3269.CHEN J Y，XIA H，HUANG K，et al. Effects of tetracycline on microbial communities and antibiotic resistance genes of vermicompost from dewatered sludge[J]. Environmental Science,2019,40(7)：3263-3269. (in Chinese)
[75] 张嘉炫. 蚯蚓堆肥对含铜猪粪中耐药基因和微生物群落的影响[D].广州：华南农业大学,2018.ZHANG J X. Effects of earthworm on antibiotic resistance genes and microbial community during copper-containing swine manure vermicomposting[D]. Guangzhou： South China Agricultural University,2018. (in Chinese)
[76] ABDELRAHMAN Y A Y，LUO Y S，SUN S S，et al. Phytochemical and underlying mechanism of Mikania micrantha Kunth on antibiotic resistance genes，and pathogenic microbes during chicken manure composting[J]. Bioresource Technology,2022,367：128241.
[77] WANG Q Z，GU J，WANG X J，et al. Effects of nano-zerovalent iron on antibiotic resistance genes and mobile genetic elements during swine manure composting[J]. Environmental Pollution,2020,258(C)：113654.
[78] MA S S，LIU H T. Three-dimensional printed bulking agents reduce antibiotic resistance genes in swine manure aerobic composting by regulating oxygen concentration to alter host microorganisms and mobile genetic elements[J]. Bioresource Technology,2022,359：127489.
[79] DAI X X，WANG X J，GU J，et al. Responses of bacterial communities and antibiotic resistance genes to nano-cellulose addition during pig manure composting[J]. Journal of Environmental Management,2021,300：113734.
[80] GUO B B，WU J P，CHEN J W，et al. Effects of Chinese medicine herbal residues on antibiotic resistance genes and the bacterial community in chicken manure composting[J]. The Journal of Antibiotics,2022,75(3)：164-171.
[81] 宝剑锋. 大孔吸附树脂对堆肥过程中抗生素抗性基因及细菌群落的影响[D].杨凌：西北农林科技大学,2020.BAO J F. Effects of macroporous adsorption resin on antibiotic resistance genes and the bacterial community during composting[D]. Yangling： Northwest A&F University,2020. (in Chinese)

Research Progress on the Removal Effect of Pathogens and Antibiotic Resistance Genes in Manure Compost of Livestock and Poultry

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[7]	XU Lifeng, ZHANG Baoge, HONG Jinxuan, WANG Xuhui, CHEN Jilong, LI Xunliang. Interaction Between Neutrophil Extracellular Traps and Pathogens and Its Underlying Mechanism [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1388-1395.
[8]	FAN Lixia, YUAN Xuexia, LI Yuanyang, WU Yuanjuan, ZHAO Shancang, ZHANG Bingchun, WANG Wenbo. LAMP Detection and Drug Resistance Analysis to Staphylococcus aureus and Escherichia coli in Livestock and Poultry Manure [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2325-2335.
[9]	WANG Fei, WANG Cailian, SONG Shuzhen, LANG Xia. Main Pathogenic Bacteria Causing Subclinical Mastitis in Yak and the Distribution of Antibiotic Resistance and Virulence Genes [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(1): 229-239.
[10]	CHEN Dishi, CHEN Bin, REN Yupeng, SHAO Jing, ZHANG Yi, WANG Zezhou. Research Pregress on New Detection Methods for the Pathogens of Swine Bacterial Infectious Diseases [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(9): 2787-2794.
[11]	CAO Dong-yang, WANG Xiao-min, MAO Ai-hua, HE Kong-wang, QIAN Ai-dong. Epidemiological Investigation of Multi-pathogens Co-infection with Post-weaning Multi-systemic Wasting Syndrome [J]. , 2015, 42(12): 3383-3391.
[12]	HAN Meng-li, KANG Li-chao, ZHONG Fa-gang, HUANG Xin, TAN Peng-fei. Research Progress on Bacterial Pathogens of the Bovine Respiratory Disease [J]. , 2013, (5): 165-172.
[13]	CHEN Fen;LIN Rui-qing;ZHU Xing-quan;LI Juan. Research Advances in Tracing Techniques for Pathogens and their Application in Parasites [J]. , 2011, 38(2): 88-92.
[14]	NI Baifeng;ZHU Jiaxin;LU Guolin;YU Guoqiao;XU Hui;ZHAO Lingyan;WANG Yong . Trends of Drugresistance and Pathogens Isolated from Animals in Zhejiang Province [J]. , 2007, 34(2): 147-149.