仔猪李斯特杆菌的分离与鉴定

doi:10.16431/j.cnki.1671-7236.2015.07.038

Abstract

Abstract: The assay was aimed to investigate the cause of death of diseased and dead piglets,we collected samples and identified by growth characteristics of bacteria in different mediums,biochemistry identification,PCR amplification of 16S rRNA genes and sequence analysis.The results showed that the isolate was Listeria.Drug sensitive test of the isolate to 20 antibiotics was detected.The results showed that this bacterium was highly sensitive to penicillin G,fosfomycin and amoxicillin and so on;However,it was resistant to furazolidone and so on.The growth curve of Listeria showed that the bacterium was in the proliferation period when 37 ℃ cultured 6 to 14 h.The results of this study provided scientific bases for detection and treatment of listeriosis in China.

Key words: piglets; Listeria; identification; 16S rRNA

CLC Number:

S852.61

LI Hao-xin, DUAN Gang, XIANG Xun, ZU Fei, YANG Zhi-yuan, ZHU Qi, DAI Fei-yan, CHANG Hua. Isolation and Identification of Listeria in Piglets[J]. , 2015, 42(7): 1883-1889.

References

[1] Crum N F.Update on Listeria monocytogenes infection[J].Curr Gastroenterol Rep,2002,4(4):287-296.
[2] 董丽丽,王春生,安笑秋,等.单核细胞增生李斯特菌简介[J].中国乡村医药,2000,12:24-25.
[3] 崔焕忠,乔立桥,王义冲.单核细胞增生性李斯特菌的主要毒力因子及其致病机理[J].中国畜牧兽医,2010,37(1):128-133.
[4] 郎需龙,王兴龙.李斯特菌毒力因子及其调控机制的研究进展[J].中国畜牧兽医,2011,38(3):195-198.
[5] Vázquez-Boland J A,Kuhn M,Berche P,et al.Listeria pathogenesis and molecular virulence determinants[J]. Clin Microbiol Rev,2001,14(3):584-640.
[6] Yucel N,Citak S,Onder M.Prevalence and antibiotic resistance of Listeria species in meat products in Ankara,Turkey[J].Food Microbiol,2005,22(2):241-245.
[7] Sergelidis D,Abrahim A.Adaptive response of Listeria monocytogenes to heat and its impact on food safety[J].Food Control,2009,20(1):1-10.
[8] Holt J G,Krieg N R,Sneath D H A,et al.Bergey's manual of determinative bacteriology[M].Ninth edition.International Edition,1994.
[9] Murray P R,Baron E J,Pfaller M A,et al.Manual of clinical microbiology[M].7th edition.American Society for Microbiology,2005.
[10] Monteiro L,Gras N,Vidal N,et al.Detection of Helicobacter pylori DNA in human feces by PCR:DNA stability and removal of inhibitors[J].J Microbiol Methods,2001,45:89-94.
[11] 王海艳,刘中学,刘虹,等.食品中单增李斯特菌快速敏感、特异PCR检测方法的建立[J].检验检疫科学,2006,1:3-6.
[12] 闫冰,李一松,霍贵成,等.食品中单核细胞增多李斯特菌的快速检测[J].食品工业科技,2006,10:202-205.
[13] 闫冰,姜毓君,曲妍妍,等.实时RT-PCR检测存活于乳中的单核细胞增多性李斯特菌[J].食品科学,2008,2:292-296.
[14] 徐伟,刘军,李素芳,等.单增李斯特菌与志贺氏菌多重PCR检测技术的建立[J].中国食品学报,2009,1:201-207.
[15] 曹玮,王宁,王晓英,等.单增李斯特菌PCR-ELISA快速检测技术研究[J].卫生研究,2009,6:662-666.
[16] 田静.熟肉制品中单增李斯特菌的风险评估及风险管理指施的研究[D].北京:中国疾病预防控制中心,2010.
[17] 程媛媛,张彦明,向华,等.牛奶中布鲁氏菌和单增李斯特菌双重PCR检测方法的建立[J].西北农林科技大学学报,2011,3:54-60.
[18] 何晓伟.可视化LAMP法快速检测半乳中单增李斯特菌的研究[D].哈尔滨:东北农业大学,2013.
[19] 童吉宇,李志清,闻一鸣,等.原核表达MurA蛋白并制备其多克隆抗体用于免疫磁珠快速检测单增李斯特菌[J].微生物学通报,2014,6:1234-1242.
[20] Lv J,Qin Z,Xu Y,et al.Listeria infection in Chinese pregnant women and neonates from Shandong[J].International Journal of Clinical and Experimental Medicine,2014,7(9):2730-2734.
[21] Day J B,Basavanna U.Real-time PCR detection of Listeria monocytogenes in infant formula and lettuce following macrophage-based isolation and enrichment[J].Journal of Applied Microbiology,2015,118(1):233-244.

Isolation and Identification of Listeria in Piglets

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