种子液传代次数对益生菌FGM株发酵黄芪转化多糖的影响

Abstract

Abstract: The assay was aimed to further improve the stability of fermentation of Astragalus membranaceus by Streptococcus alactolyticus FGM strain. First，Astragalus membranaceus-sulfuric acid method，respectively. Finally, we made a statistical analysis. The results showed that the multiple continuous passage of FGM strain had caused the serious degeneration phenomenon of FGM strain and extremely significantly affected the yield of APS （P=0.007）, so the fermentation performance was the strongest after the 4th generation of subculture. was inoculated with the successively passaged FGM strain to fermentation, then we measured the viable counts of FGM strain and the yield of Astragalus polysaccharide (APS) by bacteria plate count method and phenol The assay was aimed to further improve the stability of fermentation of Astragalus membranaceus by Streptococcus alactolyticus FGM strain. First，Astragalus membranaceus was inoculated with the successively passaged FGM strain to fermentation, then we measured the viable counts of FGM strain and the yield of Astragalus polysaccharide (APS) by bacteria plate count method and phenol-sulfuric acid method，respectively. Finally, we made a statistical analysis. The results showed that the multiple continuous passage of FGM strain had caused the serious degeneration phenomenon of FGM strain and extremely significantly affected the yield of APS （P=0.007）, so the fermentation performance was the strongest after the 4th generation of subculture.

Key words: FGM strain; passage times; fermentation; strain amount; APS content

SHANG Li-ming, QIN Zhe, ZHANG Jing-yan, WANG Xu-rong, WANG Lei, KONG Xiao-jun, CHEN Jie, YANG Zhi-qiang, WANG Xue-zhi, LI Jian-xi. Effect of FGM Strain Passage Times on the Transformation of Astragalus Polysaccharide from Astragalus membranaceus by Fermentation[J]. .

References

1 王玉阁. 微生物发酵制药的研究［J］. 齐齐哈尔医学院学报, 2006, 27(2): 196～197.
2 王龙. 发酵黄芪多糖的菌种生物学特性研究［D］. 北京: 中国农业科学院，2012.
3 王兴红，李祺德，曹秋娥. 微生物发酵中药应成为中药研究的新内容［J］.中草药, 2001，32(3): 267～268.
4 王华付. 黄芪中黄芪多糖含量的测定［J］. 安徽农业科学，2007，35(34)：10948～11021.
5 王学智，杨志强，李建喜. 发酵液中黄芪粗多糖与鸡肠道菌体外相互作用研究［J］. 甘肃农业大学学报,2008,43(6): 25～28.
6 刘屹峰. 乳酸菌的生理特性和生物学功能［J］.丹东纺专学报，2002，9(2)： 6～7.
7 朱新术，杨志强，李建喜，等. 基于神经网络和遗传算法的黄芪发酵培养基优化［J］.中兽医医药杂志，2007，3： 18～20.
8 朱新术. 发酵黄芪的乳酸菌的选育与发酵参数优化研究［D］. 北京: 中国农业科学院, 2008.
9 张凯, 杨志强, 李建喜, 等. 鸡肠内菌发酵党参转化多糖实验研究［J］.中兽医医药杂志，2008，27(3)： 5～7.
10 张凯，李建喜，杨志强，等. 发酵型黄芪提取物对肉仔鸡生产性能及血液生化指标的影响［J］. 中国畜牧兽医，2011a，38(5): 31^～34.
11 张凯，杨志强，王学智，等. 发酵型黄芪提取物对肉仔鸡生产性能及免疫球蛋白的作用研究［J］. 湖北农业科学，2011b，50(6)：1216～1218.
12 陈雷，张建梅，孙合美，等. 乳酸菌―芽孢菌发酵黄芪对小鼠免疫功能作用的研究［J］.中国畜牧兽医，2014，41(1): 126～129.
13 国春艳，杜红芳,刁其玉，等. 乳酸菌的生理功能及在畜牧业中的应用［J］. 饲料工业，2006，27(2): 55～57.
14 罗燕，谷新利，鲁芝子，等. 黄芪多糖分级组分对鸡血清中IL-2含量的影响［J］. 中国畜牧兽医，2010，37(1): 32～33.
15 范阔海，李娟娟，张蓓，等. 黄芪杂多糖对仔猪IL-6和PR-39表达的影响［J］. 中国畜牧兽医，2013，40(2): 1～5.
16 赵红霞，詹勇，许梓荣. 乳酸菌的研究及其应用［J］. 江西饲料, 2003(1): 9～12.
17 赵雯玮，陈祥贵，李鑫. 微生物发酵在中药研究中的应用［J］. 生命科学仪器，2008，6(10): 3～5.
18 彭涛，李文平，张明军，等. 黄芪多糖对力竭游泳小鼠生理生化指标的影响［J］. 中国畜牧兽医，2008，35(12): 33～36.
19 Qin Z, Li J X, Yang Z Q, et al. Effects of fermentation Astragalus polysaccharides on experimental hepatic fibrosis［J］. Journal of Animal and Veterinary Advances, 2012, 11: 1195～1203.

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Effect of FGM Strain Passage Times on the Transformation of Astragalus Polysaccharide from Astragalus membranaceus by Fermentation

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