Abstract: To study cell apoptosis changes induced by different concentrations of aphidicolin, we used flow cytometry to get Jurkat cell apoptosis rates. We found that dose and time dependent effects appeared because of Jurkat cell apoptosis induced by aphidicolin. With the increasing of aphidicolin concentrations or incubation time, the early apoptosis rate, late apoptosis rate and total apoptosis rate all increased. There was an obvious increasing trend when the concentration of aphidicolin was more than or equal to 1 μg/mL, or the incubation time was more than or equal to 16 hours.

Key words:

aphidicolin; flow cytometry; cells apoptosis