油酸和硬脂酸对奶牛乳腺上皮细胞中脂肪酸结合蛋白3表达和脂滴分泌的影响

Abstract

Abstract: To study the effect of oleic acid and stearic acid on the expression of fatty acid binding protein 3 (FABP3) in dairy cow mammary epithelial cells, qRT-PCR was used to detect the expression of genes related with milk fat synthesis. The optimal concentrations and time of oleic acid and stearic acid were 75 μmol/L 12 h and 100 μmol/L 36 h, respectively. Western blotting and immunofluorescence were used to detect the expression of FABP3 and secretion of lipid droplets. These data provided further experimental evidence for studying the effect of FABP3 on the milk fat synthesis signal transduction pathway.

Key words: oleic acid; stearic acid; dairy cow mammary gland epithelial cells; fatty acid binding protein 3; lipid droplets

LIANG Meng-yao, GAO Xue-jun, HOU Xiao-ming, LI Qing-zhang. Effect of Oleic Acid and Stearic Acid on the Expression of Fatty Acid Binding Protein 3 and Secretion of Lipid Droplets in Dairy Cow Mammary Epithelial Cells[J]. .

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Effect of Oleic Acid and Stearic Acid on the Expression of Fatty Acid Binding Protein 3 and Secretion of Lipid Droplets in Dairy Cow Mammary Epithelial Cells

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