油酸和亚油酸对山羊乳腺细胞甘油三酯含量及乳脂合成相关基因表达的影响

doi:10.16431/j.cnki.1671-7236.2019.02.007

Abstract

Abstract:

The aim of this study was to investigate the effects of oleic acid and linoleic acid on triglyceride (TG) content and the expression of genes involved in milk fat synthesis in dairy goat mammary epithelial cells in vitro.The goat mammary epithelial cells were used as the research object,and different concentrations of oleic acid (0,50,100,200 μmol/L) and linoleic acid (0,20,40,80 μmol/L) were added in culture medium for a 24 h cultivation.The intracellular TG content was measured by using triglyceride determination kit,and gene expressions were detected by Real-time quantitative PCR.The results showed that 100,200 μmol/L oleic acid and 40,80 μmol/L linoleic acid significantly increased TG content (P<0.05).The expression of DGAT2,ACC and FASN genes were significantly up-regulated by addition of 100 and 200 μmol/L oleic acid (P<0.05),while the SCD1 gene expression was significantly inhibited by oleic acid (P<0.05).However,oleic acid had no significant effect on the expression of PPARγ gene (P>0.05).The results of linoleic acid addition showed that 20 μmol/L linoleic acid could significantly inhibit the expressions of ACC and FASN genes (P<0.05).40 and 80 μmol/L linoleic acid could significantly promote the expression of DGAT2 gene (P<0.05),inhibit the expression of SCD1 and SREBP1 genes (P<0.05),and had no significant effect on the expression of ACC,FASN and PPARγ genes (P>0.05).In conclusion,100 to 200 μmol/L oleic acid and 40 to 80 μmol/L linoleic acid were optimal levels considering its improvement effects on milk fat synthesis in dairy goat mammary epithelial cells.

Key words: oleic acid; linoleic acid; goat mammary epithelial cells; triglyceride(TG); genes expression

CLC Number:

S827

LI Jun, YANG Wenzhuo, HOU Xiafei, WANG Xiaoxiao, JI Xiangbo, LI Xinfeng, QUAN Kai. Effects of Oleic Acid and Linoleic Acid on Triglyceride Content and Gene Expression Involved in Milk Fat Synthesis in Goat Mammary Epithelial Cells[J]. , 2019, 46(2): 380-386.

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Effects of Oleic Acid and Linoleic Acid on Triglyceride Content and Gene Expression Involved in Milk Fat Synthesis in Goat Mammary Epithelial Cells

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