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Study on the Extraction Process of Compound Epimedium by Orthogonal Experimental Design

GU Yu-feng1, HUANG Ya-jun1, WANG Qin1, WU Yong1, LIU Zhao-ying1, ZHANG Ming-jun1, SUN Zhi-liang2, JIANG Zheng-yun3   

  1. (1.Veterinary Facultry, Hunan Agricultural University, Changsha 410128, China;2.Hunan Engineering Research Center of Veterinary Drug, Changsha 410128,China;3.Animal Health Engineering and Technology Research Center, Hunan Agricultural University, Changsha 410128,China)
  • Received:2013-07-11 Online:2014-03-20 Published:2014-05-15

Abstract: The assay was aimed to optimize the extraction process of the compound Epimedium by orthogonal experimental design. The extraction process of the compound Epimedium was optimized with water volume, extraction times and extraction time as independent variables. The extraction ratio of icariin was choosed as an evaluation index. The optimized extraction conditions were as follows, extracted in 10 times water (solid-liquid rate at 1∶10) for 3 times and heated for 1.5 h per time. These results indicated that the optimum extraction technology by the orthogonal experimental design provided experimental bases for improving the content of icariin and its industrial production.

Key words: compound Epimedium; icariin; orthogonal experimental design; extraction process