Abstract: This study was aimed to investigate α-mannosidase (AMA) gene transcription at different time by intragastric drenching different doses of swainsonine (SW) to mice. Animal total RNA extraction kit, microplate method, gel electrophoresis and Real-time quantitative PCR were used in this experiment to extract liver total RNA and detect RNA purity, RNA integrity and AMA gene transcription. The results showed that AMA gene of the experimental groups and control group were expressed and different experimental groups had different transcription levels. Compared with control group, low doses of SW could promote AMA gene expression and the promotion gradually increased as the trial went on. However, high and middle doses of SW could promote AMA gene expression in initial stage and inhibit it seriously in long time and the inhibition gradually increased as the trial went on. The results showed that different doses of SW had effect on mouse liver AMA gene transcription and expression at different time and different levels.

Key words: swainsonine; α-mannosidase gene; Real-time quantitative PCR