致犊牛肺炎牛支原体南疆株的分离鉴定及oppF基因序列分析

Abstract

Abstract: This study was to identify the pathogen from calves which showed pneumonia symptoms occurred in a cattle farm of Kashi city in the South of Xinjiang. Three lung specimens were collected from the dead calves. Two isolates (designated as M.bovis-NJ-1 and M.bovis-NJ-2) were isolated from three pneumonic lung tissue samples of calves with Mycoplasma bovis special liquid medium and solid medium. Both isolates were identified as Mycoplasma bovis by the morphologic observation and specific PCR test, respectively. Colonies of two isolates growed on solid medium were typical fried-egg shape and Dienes staining was dark blue. An expected gene fragment of 448 bp specific for Mycoplasma bovis was amplified by PCR. In addition, the oppF genes of the isolates were amplified and sequenced, the homologies of which were 96.7% and 95.3% with reference strain Mycoplasma bovis PG45 available in the GenBank, respectively. The results indicated that Mycoplasma bovis was responsible for the calf pneumonia.

Key words: mycoplasma pneumonia of calf; Mycoplasma bovis; isolation; PCR identification; oppF

CLC Number:

S858.23

YANG Ming-wei, LI Di, ZHAO Hai-long, YAN Gen-qiang, WANG Jing-mei. Isolation and Identification of Mycoplasma bovis Nanjiang Strain Inducing Pneumonia and Sequence Analysis of oppF Gene[J]. , 2014, 41(12): 25-29.

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Isolation and Identification of Mycoplasma bovis Nanjiang Strain Inducing Pneumonia and Sequence Analysis of oppF Gene

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