不同日龄阿勒泰大尾羊脂肪组织形态学及血脂指标的比较

Abstract

Abstract: In order to study the variation in different parts of adipose tissues deposition of Aletai sheep, 90 days (n=6) and 270 days (n=6) old ram of Aletai sheep were chosen in this study.The adipocyte area of perirenal fat, tail fat and subcutaneous fat were measured by using the method of oil red O staining on adipose tissue frozen section and Motic microscopic digital image processing system.Serum lipid metabolism related enzymes and hormones were measured by using radioimmunoassay and ELISA method. The results showed that in 90 days old Aletai sheep, the adipocyte area of tail fat was very significantly higher than adipocyte area of perirenal fat (P<0.01), in 270 days old Aletai sheep, adipocyte area of tail fat and subcutaneous fat were very significantly higher than adipocyte area of perirenal fat (P<0.01). Compared with 90 days old Aletai sheep, adipocyte area of perirenal fat in 270 days old Aletai sheep was significantly increased (P<0.05), and adipocyte area of tail fat was very significantly decreased (P<0.01). Serum leptin and hormone semitive lipase (HSL) concentrations of 270 days old Aletai sheep were very significantly lower than that of 90 days old Aletai sheep (P<0.01), and concentrations of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were very significantly higher (P<0.01). These results indicated that variation in different parts of adipose tissues deposition of different old Aletai sheep were inconsistent, this change might be related to serum leptin, HSL, AST and ALT levels.

Key words: Aletai sheep; adipose tissue; morphology; serum lipids

CLC Number:

S826.9⁺5

WANG Jin-quan, WANG Xiao-yan, ZANG Chang-jiang, LIU Wu-jun, PENG Hua-gang, WANG Qi, YUE Xiao-fan, YAO Gang. Comparison of the Serum Lipids Index and Morphology of Adipose Tissue in Different Old Aletai Sheep[J]. , 2014, 41(12): 167-171.

References

1 王金泉,王肖燕,叶青,等.阿勒泰大尾羊与小尾寒羊不同组织FTO基因的检测[J].动物医学进展,2013,12:84～88.
2 王春艳.正常人群中ALT、AST血清水平及PLT水平的参考值评估[D].长春:吉林大学,2008.
3 文旭辉,罗桂芬,孙世铎,等.Leptin对猪成肌细胞中PGC-1和UCPs mRNA表达的影响[J].农业生物技术学报,2007,4:644～648.
4 朱庆龄,叶新华,杨声坪,等.学龄期肥胖儿童肝X受体与脂代谢异常的相关性分析[J].临床儿科杂志,2014,2:147～150.
5 乔永,黄治国,李齐发,等.绵羊肌肉中FAS基因和HSL基因的发育性变化及其对肌内脂肪含量的影响[J].遗传学报,2007,10:909～917.
6 刘华珍,彭克美.Leptin及其受体的研究进展[J].华中农业大学学报,2003,22(1):90～94.
7 刘琼,肖新华.皮下脂肪组织与内脏脂肪组织[J].国际病理科学与临床杂志,2013,6:544～547.
8 杨晓慧.猪MC4R和ME1基因的多态性及其与背膘厚的关系研究[D].泰安:山东农业大学,2008.
9 杨雪芬,蒋宗勇.瘦素对猪脂肪沉积与代谢调控的研究进展[J].中国畜牧兽医,2009,36(8): 18～22.
10 杨海玲,曾勇庆,魏述东,等.莱芜猪脂肪代谢酶活性的发育性变化及其对肌内脂肪沉积的影响[J].畜牧兽医学报,2005,36(11):43～47.
11 张冰,孙超,黄敏瑞,等.陆川猪Leptin基因的SNP检测及其与部分生产性状的关联分析[J].西北农业学报,2007,5:26～29.
12 Hoehne A,Nuemberg G,Kuehn C,et al.Relationships between intramuscular fat contents,selected carcass traits,and fatty acid profile in bulls using a F2-population[J].Meat Science,2012,90:629～635.
13 Keith N F,Simon W C,Barbara A F,et al.Coordinated regulation of HSL and LPL in human adipose tissue in vivo: Implications for the control of fat storage and fat mobilization[J].Enzyme Regul,1995,35:163～178.
14 Morris C A,Cullen N G,Glass B C,et al.Fatty acid synthase effects on bovine adipose fat and milk fat[J].Mammalian Genome,2007,1(18):64～74.
15 Pan G Y,Fu B,Zuo Z,et al.Molecular-characterization,expression profile and association analysis with fat deposition traits of the porcine APOM gene[J].Mol Biol,2010,37:1363～1371.
16 Wood J D,Enser M,Fisher A V,et al.Fat deposition,fatty acid composition and meat quality: A review[J].Meat Science,2008,78:343～358.
17 Yamauchi T,Kamon J,Minokoshi Y,et al.Adiponectin stimulates glucose utilization and fatty-acid oxidation by activating AMP-activated protein kinase[J].Nat Med,2002,8(11):1288～1295.
18 Zhang Y,Ricardo P,Margherita M,et al.Poditional cloning of the mouse obese gene and its human homologue[J].Nature,1994,372:425～432.

[1]	CHANG Yitong, ZHANG Wei, PENG Yinglin, CHEN Chen. Evolutionary Analysis,Target Gene Prediction and Tissue Expression Analysis of miR-192 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 882-892.
[2]	FANG Qinyuan, FU Shaoyin, WANG Biao, HE Xiaolong, HE Jiangfeng, WANG Liwei, CHEN Xin, ZHANG Lin, WANG Daqing, MA Yuejun, ZHANG Liling, LIU Yongbin. Research Progress on Influencing Factors of Brown Fat Activity in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1059-1067.
[3]	LIU Zhenni, LEI Xiaowen, LI Jianjun, ZENG Qingyuan, TAN Donghai, ZHONG Yunping, ZHANG Qiang, XIE Hualiang. Effects of Fermented Navel Orange Pomace on Intestinal Morphology,Intestinal Mucosal Immune Function and Microbial Diversity of Muscovy Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 573-585.
[4]	WANG Shuaijie, TAN Zihan, LI Hanbo, SUN Xiangxue, AI Sihan, WANG Lei, ZHAO Di, HOU Yongqing. Effects of Quercetin on Intestinal Morphology, Antioxidant Function and Jejunal Lipid Metabolism Gene Expression in Piglets with Porcine Epidemic Diarrhea Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3391-3399.
[5]	PENG Kai, CHEN Bing, ZHAO Hongxia, WANG Lijuan, ZHU Xifeng, HUANG Wen. Effects of Condensed Tannins on Growth Performance,Body Composition,Apparent Digestibility of Nutrients,and Intestinal Morphology of Lateolabtax maculatus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2953-2960.
[6]	YU Xiaojun, LI Dandan, LI Xuewu, WANG Qun, WANG Guang, WANG Lihua. Effects of Antibacterial Peptide on the Immune Function and Intestinal Tissue Morphology of Growing Female Minks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 443-453.
[7]	WANG Jiaxin, HAN Yunsheng, CAI Hongying, WEN Zhiguo, XU Xin, ZHAO Zitao, MENG Kun, YANG Peilong. Effects of Bacillus licheniformis on Growth Performance, Serum Biochemical Indexes and Intestinal Morphology of Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4593-4603.
[8]	WANG Lizhong, WU Tiecheng, MA Yuejun, LI Yurong, WU Yahan, HE Tuoya, ZHAO Shengguo, LIU Bin. Expression Analysis of Histomorphology,chi-miR-107-3p and RHBDF 2 Genes of Hair Follicle in Cashmere Goats at Anagen Prosperity [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 1918-1926.
[9]	HUO Miao, LI Yanan, YANG Sirui, ZHU Xiangde, XU Xiaofeng, ZHANG Lili. Effect of Feeding Methods on Fatty Acid Composition of Muscle and Adipose Tissue in Tan Sheep Lamb [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2020-2032.
[10]	LI Wenjing, WEI Kaimin, ZHANG Shaoqiu, CHEN Yong. Effects of Walnut Green Husk and Its Extract on Intestinal Morphology,Mucosal Antioxidant Activity and Microbial Diversity of Yellow-feather Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2056-2065.
[11]	LI Xiang, HE Jinhuan, PAN Chunmei, SUO Jianghua, LI Jing. Research Progress on Effect of Sodium Butyrate on Intestinal Morphology, Digestion and Absorption Function of Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1603-1612.
[12]	ZHANG Liping, WANG Jianfeng, LI Zhenzhen, LEI Qiong, DANG Haoqian, LIU Qinghua. Effects of Different Sources of Fiber Diet on Apparent Digestibility,Duodenal Tissue Morphology and Cecal Fiber Digestive Enzyme Activity in Fujian Yellow Rabbits [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 501-508.
[13]	WU Zhengjiao, HOU Linjing, RAO Guoshun, JIN Weikun, FANG Fang, ZHANG Weiyu, DI Wenda. Identification of Paramphistomidae in the Liver of Buffalos in Nanning,Guangxi [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 668-675.
[14]	PAN Jianfei, ZHENG Qiantao, TAO Cong, ZHAO Jianguo, WANG Yanfang. Comparison of Lipid Profiles of Backfat and Inguinal Subcutaneous White Adipose Tissue from Acute Cold-treated Bama Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 2759-2766.
[15]	TAO Zhenyang, ZHANG Yaowen, CAO Zhongyang, JIAO Guobao, ZHANG Zhidan, LIU Fangyuan, WANG Jie, WANG Zhanbin. Effects of Enterococcus faecalis on Production Performance,Egg Quality and Intestinal Morphology of Laying Hens During the Late Laying Period [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1413-1420.

Comparison of the Serum Lipids Index and Morphology of Adipose Tissue in Different Old Aletai Sheep

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments