›› 2014, Vol. 41 ›› Issue (12): 109-116.

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Development of High-throughput Sequencing Technology and its Application in Bioscience

DU Ling1,2, LIU Gang2, LU Jian2,3, LIU Chou-sheng2, HA Fu1   

  1. 1. Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650000, China;
    2. National Center for Preservation and Utilization of Animal Genetic Resources, National Animal Husbandry Service, Beijing 100193, China;
    3. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
  • Received:2014-06-05 Online:2014-12-20 Published:2014-12-25

Abstract: The DNA sequencing technology, developing from the first generation to high-throughput sequencing (HTS) technology, has experienced a long and tortuous process amazing progress now. The technology has been used widely in the animal and plant genome with a great success. In addition, its also makes us to investigate the whole genome with lower cost, more in-depth. In this study, we had elaborated sequencing plaforms of HTS, such as Roche/454 sequencing, ABI/SOLID sequencing, Illumina/Solexa sequencing, single molecule sequencing and Ion Torrent sequencing technology. And we also had summarized the application of HTS in the genomics, transcriptomes, and epigenetics.

Key words: high-throughput sequencing; genomics; transcriptomes; application

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