奶牛源化脓隐秘杆菌的分离鉴定及其毒力基因的检测

Abstract

Abstract: The purpose of this study was to identify the strain CVCC 3982 isolated from a death dairy cow's lung in Beijing and determine its pathogenicity. The suspected pathogen was identified by means of Biolog system and 16S rDNA sequence analysis. Its pathogenicity was determined by artificial inoculation in CD-1 mice, synthetic primers were used to examine the main virulence genes. The results showed that the suspected pathogen was gram positive bacillus, β hemolysis. The identification result of Biolog was Trueperella pyogenes. The homology of 16S rDNA gene sequence with the Trueperella pyogenes type strain NCTC 5224 reached 100%. By the phylogenetic analysis, the isolate was in the same branch with Trueperella pyogenes. The isolate contained hemolysin (plo) gene, neuraminidase H (nanH) gene, neuraminidase P (nanP) gene, fimbriae gene (fimA, fimC, fimE), but was lack of collagen-binding protein (cbpA) gene and fimG gene. Infection of suspected bacteria resulted in mortality to mice by intraperitoneal injection. The results indicated that the isolate was a virulent Trueperella pyogenes.

Key words: dairy cow; Trueperella pyogenes; Biolog; 16S rDNA; virulence genes

CLC Number:

S852.61

LI Wei-jie, WEI Cai-wen, LIU Yan, QI Xiao-xin, TIAN Ye, JIANG Tao-zhen. Isolation, Identification of Trueperella pyogenes from Dairy Cow and Detection of Major Virulence Genes of the Isolate[J]. , 2014, 41(11): 68-72.

References

1 李伟杰,赵耘,杜昕波,等. 动物园野生动物支气管败血波氏杆菌的分离鉴定与系统发育树的构建[J]. 中国兽医学报,2010,30(3):344～346.
2 鲁杏花,何世成,谈志祥,等. 猪化脓隐秘杆菌的分离与鉴定[J]. 中国畜牧兽医,2009,36(5):149～152.
3 Aghamiri S M, Haghkhah M, Ahmadi M R, et al. Development of a multiplex PCR for the identification of major pathogenic bacteria of post-partum endometritis in dairy cows[J]. Reprod Domest Anim, 2014, 49(2): 233～238.
4 Amoes M R, Healey G D, Goldstone R J, et al. Differential endometrial cell sensitivity to a cholesterol-dependent cytolysin links Trueperella pyogenes to uterine disease in cattle[J]. Biology of Reproduction, 2014, 90(3): 1～13.
5 Confer A W. Update on bacterial pathogenesis in BRD[J]. Anim Health Res Rev, 2009, 10(2): 145～148.
6 Goldstone R J, Amos M, Talbot R, et al. Draft genome sequence of Trueperella pyogenes, isolated from the infected uterus of a postpartum cow with metritis[J]. Genome Announcements, 2014, 2(2): e00194～e00214.
7 Jost B H, Songer J G, Billington S J. An Arcanobacterium (Actinomyces) pyogenes mutant deficient in production of the pore-forming cytolysin has reduced virulence[J]. Infect Immun, 1999, 67(4): 1723～1728.
8 Jost B H, Songer J G, Billington S J. Cloning, expression and characterization of a neuraminidase gene from Arcanobacterium pyogenes[J]. Infec Immu, 2001, 69(7): 4430～4437.
9 Jost B H, Songer J G, Billington S J. Identification of a second Arcanobacterium pyogenes neuraminidase and involvement of neuraminidase activity in host cell adhesion[J]. Infec Immu, 2002, 70(3): 1106～1112.
10 Machado V S, Bicalho R C. Complete genome sequence of Trueperella pyogenes, an important opportunistic pathogen of livestock[J]. Genome Announcements, 2014, 2(2): e00400～e00414.
11 Pietrocola G, Valtulina V, Rindi S, et al. Functional and structural properties of CbpA, a collagen binding protein from Arcanobacterium pyogenes[J]. Microbiology, 2007, 153 (Pt 10): 3380～3389.
12 Rzewuska M, Stefańska I, Osińska B, et al. Phenotypic characteristics and virulence genotypes of Trueperella (Arcanobacterium) pyogenes strains isolated from European bison (Bison bonasus)[J]. Veterinary Microbiology, 2012, 160(1～2): 69～76.
13 Santos T M A, Caixeta L S, Machado V S, et al. Antimicrobial resistance and presence of virulence factor genes in Arcanobacterium pyogenes isolated from the uterus of postpartum dairy cows[J]. Veterianry Microbiology, 2010, 145(1～2): 84～89.
14 Silva E, Gaivo M, Leito S, et al. Genomic characterization of Arcanobacterium pyogenes recovered from the uterus of dairy cows with normal puerperium or clinical metritis[J]. Vet Microbiol, 2008, 132(1～2): 111～118.
15 Yassin A F, Hupfer H, Siering C, et al. Comparative chemotaxonomic and phylogenetic studies on the genus Arcanobacterium Collins et al. 182 emend. Lehnen et al. 2006: Proposal for Trueperella gen. nov. and emended description of the genus Arcanobacterium[J]. International Journal of Systematic and Evolutionary Microbiology, 2011, 61(6): 1265～1274.
16 Zastempowska E, Lassa H. Genotypic characterization and evaluation of an antibiotic resistance of Trueperella pyogenes (Arcanobacterium pyogenes) isolated from milk of dairy cows with clinical mastitis[J]. Veterinary Microbiology, 2012, 161(1～2): 153～158.
17 Zhang W L, Meng X L, Wang J W. Sensitive and rapid detection of Trueperella pyogenes using loop-mediated isothermal amplification method[J]. Journal of Microbiological Methods, 2013, 93(2): 124～126.
18 Zhao K L, Tian Y Q, Yue B S. Virulence determinants and biofilm production among Trueperella pyogenes recovered from abscesses of captive forest musk deer[J]. Archives of Microbiology, 2013, 195(3): 203～209.

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Isolation, Identification of Trueperella pyogenes from Dairy Cow and Detection of Major Virulence Genes of the Isolate

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