蒙古绵羊肌内脂肪沉积相关基因实时荧光定量PCR检测方法的建立

Abstract

Abstract: This experiment was conducted to establish a detection method which was suitable for genes expression of Mongolia sheep intramuscular fat (IMF) deposition genes. According to the sequences of IMF deposition genes in GenBank, pairs of specific primers were designed and synthesized. Then the amplified fragments were detected by cloning technology to ensure the accuracy of the PCR products. The Real-time fluorescent quantitative PCR (Real-time PCR) was used to check the feasibility of method. Results showed that Real-time PCR optimum cycle parameters were 94 ℃ 4 min, 94 ℃ 30 s, 58 ℃ 45 s, 72 ℃ 1 min,40 cycles;72 ℃ 5 min.Each peak melting curve was a single gene, the target product was stable. The method of Real-time PCR was established successfully and could be specially used to detect IMF deposition genes of Mongolia sheep.

Key words: Mongolia sheep; fat deposition; Real-time PCR; establishment method

CLC Number:

Q344.⁺13

MIAO Hai-ming, GU Yue, YANG Jin-li, GAO Ai-wu, HUANG Ya-juan, WANG Hai-rong. Establishment of Real-time PCR Assays for Detection of the Intramuscular Fat Deposition Related Genes in Mongolia Sheep[J]. , 2013, 40(9): 37-41.

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Establishment of Real-time PCR Assays for Detection of the Intramuscular Fat Deposition Related Genes in Mongolia Sheep

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