阿勒泰羊生长速度与主要血液生化指标含量及尾脂Leptin基因表达关系

doi:10.16431/j.cnki.1671-7236.2021.07.011

Abstract

Abstract: The purpose of the experiment was to investigate the relationship between the growth rate of Altay sheep and the fat deposition by studying the content of main blood biochemical indicators and the expression level of the Leptin gene in the tail fat of Altay sheep at different growth rates.300 Altay rams with similar birth times were selected and fed normally to 6 months of age.During this process,the daily weight gain was recorded.Then the sheep whose daily weight gain was top 10% was defined as the fast growth group (the group with higher body weight,HBW),and those whose daily weight gain was bottom 10% was devided into the slow growth group (the group with lower body weight,LBW).From the fast-growing group,12 sheep were randomly selected and divided into growth inhibition group (low-energy diets,HBW-75%,n=6) and control group (standard diet,HBW-100%,n=6).In the slow growth group,12 sheep were randomly selected into the growth promotion group (high-energy diets,LBW-125%,n=6) and the control group (standard diet,LBW-100%,n=6).After 7 d of prefeeding,the sheep were fed for another 30 d,at the end of feeding,the sheep were weighted and the blood were collected,and tail fat was collected after slaughter.The levels of TG,CK,TSH,T3,T4,S.S,GH,INS,PG,Leptin in blood,the expression of Leptin mRNA and protein,and the morphological changes of tail fat cells were measured.The weight results showed that the initial weight and final weight of the HBW-100% and LBW-100% groups were significantly different (P<0.05),HBW-75% and HBW-100% groups had no significant difference in initial body weight and final body weight (P>0.05);LBW-100% and LBW-125% groups had significant difference in initial body weight (P<0.05),but no significant difference in final body weight (P>0.05).The T4,S.S,Leptin,TSH and T3 content in the LBW-100% group were significantly lower than those in the HBW-100% group (P<0.05).The T3 content in the LBW-125% group was significantly higher than that in the LBW-100% group (P<0.05).The content of GH,PG and Leptin in the HBW-75% group were significantly lower than that in the HBW-100% group (P<0.05).The tail fat cells in the LBW-125% group were larger than that in the LBW-100% group,and the HBW-75% group was smaller than the HBW-100% group (P<0.05).The detection results of Leptin mRNA and protein expression levels showed that there was no significant difference between the Leptin mRNA expression levels in the HBW-100% group and the LBW-100% group (P>0.05),and the Leptin protein expression levels were significantly higher than that in the LBW-100% group (P<0.01).The expression of Leptin mRNA and protein in the LBW-125% group were significantly higher than that in the LBW-100% group (P<0.05).The expression of Leptin mRNA in the HBW-75% group was significantly lower than that in the HBW-100% group (P<0.01),and the expression of Leptin protein was significantly lower than that in the HBW-100% group (P<0.05).In summary,in the fast-growing group and the slow-growing group,the expression of Leptin had a certain connection with body weight and body fat.In the slow-growing group,body weight and fat were increased significantly after promoting growth,and the expression of Leptin showed an upward trend.In the fast-growing group,after inhibiting growth,the body weight and fat were decreased,and the expression of Leptin showed a downward trend.The change of growth rate would affect the expression of Leptin,thereby changing the fat deposition.

Key words: Altay sheep; Leptin; fat deposition

CLC Number:

S852.2

MAIMAITIYIMING·Maimaitili, YE Mengjun, HOU Meng, WANG Junli, TANG Anqi, XIANG Weiwei, LIU Luo, WANG Jinquan, HU Yan. The Relationship Between the Growth Rate of Altay Sheep and the Content of Main Blood Biochemical Indexes and the Expression of Leptin Gene in Tail Fat[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2369-2377.

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The Relationship Between the Growth Rate of Altay Sheep and the Content of Main Blood Biochemical Indexes and the Expression of Leptin Gene in Tail Fat

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