一株兔源耐药性肺炎克雷伯氏菌的分离与鉴定

Abstract

Abstract: A suspected pathogen was newly isolated from rabbits with diarrhea and respiratory tract infection by isolation and culture. To further understand some features of this suspected bacteria, microscopic examination, biochemical detection including capsular stain, drug sensitive test and 16S rDNA sequence analysis were performed. The results showed that the isolated bacteria was in rod containing rich capsular polysaccharide, and demonstrated a significant multi-drug resistance to streptomycin, gentamicin, ampicillin, gentamicin sulfate, kanamycin sulfate and chloramphenicol at different levels. Furthermore 16S rDNA sequence of this suspected bacteria was cloned by PCR and sequenced, the result showed that 16S rDNA sequence shared about 99% similarity to that of K.pneumonia standard strains provided by GenBank. In addition, infection of suspected bacteria (10⁹ CFU/mL) resulted in mortality to rabbits by intraperitoneal injection, but not by oral administration. Altogether, it could be concluded that the isolated bacteria was a member of K.pneumonia and contributed to the disease of rabbits.

Key words: K.pneumonia; drug resistance; isolation; identification

CLC Number:

S852.61

ZHANG Zhi, LI Zai-xin, ZENG Guang-ju, LI Bing-chao, HU Wei-dong, ZHAO Zhi-ping, LIU Zhang-qin, CHEN Yu-yun. Isolation and Identification of a Resistant K.peneumoniae Strain from Rabbits[J]. , 2013, 40(9): 199-203.

References

1 马磊, 颜其贵, 万莉,等. 竹鼠肺炎克雷伯氏菌的分离鉴定[J]. 中国人兽共患病学报, 2011, 27(9): 825~827.
2 王亨, 孟霞, 邱昌伟, 等. 肺炎克雷伯氏菌对荷斯坦奶牛乳腺上皮细胞黏附和侵袭的体外研究[J]. 畜牧兽医学报, 2008, 39(4): 494~498.
3 王孝友, 杨睿, 沈克飞, 等. 兔肺炎克雷伯氏菌的分离与鉴定[J]. 中国兽医杂志, 2012, 48(2): 43~44.
4 赵波,李波,王强, 等. 4个动物园灵长类动物肠道克雷伯氏菌的分离鉴定与药敏试验[J]. 中国畜牧兽医, 2013, 40(2): 161~165.
5 杨春华, 曹际娟, 钟毅, 等. 乳及乳制品中肺炎克雷伯氏菌PCR-DHPLC检测新技术的建立[J]. 微生物学通报, 2010, 37(12): 1805~1810.
6 Brisse S, Duijkeren E. Identification and antimicrobial susceptibility of 100 Klebsiella animal clinical isolates[J]. Veterinary Microbiology,2005,105(3~4): 307~312.
7 Didelot X, Falush D. Inference of bacterial microevolution using multilocus sequence data[J]. Genetics,2007, 175(3): 1251~1266.
8 Kit B, Dennis S H. Sequencing of 16S rDNA of Klebsiella: Taxonomic relations within the genus and to other Enterobacteriaceae[J]. International Journal of Medical Microbiology, 2003, 292(7): 495~503.
9 Kohler J E, Hutchens M P, Sadow P M, et al. Klebsiella pneumoniae necrotizing fasciitis and septic arthritis: An appearance in the Western hemisphere[J]. Surgical Infections (Larchmt), 2007, 8(2): 227~232.
10 Lai Y C,Yang S L,Peng H L, et al. Identification of genes present specifically in a virulent strain of Klebsiella pneumonia[J]. Infection and Immunity, 2000, 68(12):7149~7151.
11 Lu C H, Chang W N, Chang H W. Klebsiella meningitis in adults: Clinical features, prognostic factors and therapeutic outcomes[J]. Journal of Clinical Neuroscience, 2002, 9(5): 533~538.
12 Melano R, Corso A, Petroni A, et al. Multiple antibiotic-resistance mechanisms including a novel combination of extended-spectrum β-lactamases in a Klebsiella pneumoniae clinical strain isolated in Argentina[J]. Journal of Antimicrobial Chemotherapy, 2003,52(1): 36~42.
13 Podschun R, Ullmann U. Klebsiella spp.as nosocomial pathogens: Epidemiology, taxonomy, typing methods, and pathogenicity factors[J]. Clinical Microbiology Reviews, 1998, 11(4): 589~603.
14 Wong C H, Kurup A, Wang Y S, et al. Four cases of necrotizing fasciitis caused by Klebsiella species[J]. European Journal of Clinical Microbiology & Infectious Diseases, 2004, 23(5): 403~407.

[1]	LU Yukui, LIU Jiaqi, ZHONG Jiacheng, CHEN Jidang, ZHU Wanjun, ZHANG Yishan, ZHANG Jipei. Isolation,Identification and Pathogenicity Analysis of Avibacterium paragallinarum from Geese [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2562-2572.
[2]	PENG Yangxiang, YANG Zhenbiao, YAN Kuiyou, WANG Han, HU De, WANG Zun, LIU Ning, ZHAO Liansheng. From Artificial to Intelligent:Research Progress of Individual Idendification Technology for Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1855-1866.
[3]	ZENG Chengrong, LIU Xin, BI Wenwen, MEI Shihui, HE Guangxia, ZHANG Junjie, WEN Ming, ZHOU Bijun, CHEN Jiangfeng, JIANG Haibo. Research Progress on Quorum Sensing System of Aeromonas hydrophila and Its Inhibitor Application [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1991-2000.
[4]	LI Zhijie, ZHAO Ying, MA Xin, WANG Meng, YU Sijiu, WANG Libin, PAN Yangyang. Research Progress on Isolation Methods and Main Reproductive Functions of Exosomes from Animal Follicular Fluid [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1480-1488.
[5]	TIAN Changqing, ZHANG Kunzhong, QI Yumei, SHI Wenjing, DONG Zhijie, ZHANG Haohao, HE Zengwen, ZHI Ji, ZHAO Xuehui, CHONG Qian, XUE Huiwen, GOU Huitian. Isolation,Identification and Biological Characteristics Analysis of a Strain of Listeria monocytogenes from Environment [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1543-1555.
[6]	WANG Lucai, SALANG Wenzhu, ZHANG Huanrong. Isolation,Identification and Drug Resistance and Pathogenicity Analysis of Salmonella Enteritidis from Avian [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1663-1674.
[7]	WU Zhi, ZHANG Cong, YUAN Huisha, DONG Hongyan, DAI Lixia, JIA Luqi, WU Shuang, ZHU Shanyuan. Isolation,Identification,Virulence and Drug Resistance of Salmonella from Yellow Chicken [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1695-1706.
[8]	CAO Yingying, LIANG Liang, ZENG Yirong, ZHONG Qinghua, YUAN Xiaofang, TANG Haibo, LENG Jing. Isolation,Identification and Biological Characteristics of Salmonella from Tupaia belangeri chinensis [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1718-1728.
[9]	ZHU Qiqi, ZHANG Jiyu, WANG Chengye, WU Lingyu, WANG Weiwei, ZHU Zhen. Analysis of Multidrug Resistance and Prevalence of Animal-derived Escherichia coli in Hebei [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1081-1092.
[10]	LIU Kexin, WANG Chao, ZHANG Ao, LIU Jiali, TAN Bin, ZHANG Shuqin. Isolation,Identification and Genome Sequence Analysis of Bovine Viral Diarrhea Virus LN-1 Strain [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1150-1159.
[11]	CHEN Dong, ZHANG Yanhao, ZHANG Yang, LI Sen, ZHOU Zutao, LI Zili. Isolation,Identification and Drug Sensitivity of 3 Strains of Riemerella anatipestifer [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1250-1258.
[12]	HU Yifan, MA Jinghua, BAI Siqi, ZHAI Yimeng, LIU Jianxun, SONG Houhui, YANG Yongchun. Isolation,Identification and Biological Characteristics Analysis of Morganella morganii from Endometritis of South China Tiger (Panthera tigris amoyensis) [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1271-1278.
[13]	WANG Bingyi, MA Hongcai, ZOU Minghao, FAN Shijie, YUAN Zhenjie, Labaciren, DONG Hailong, ZENG Jiangyong. Isolation,Identification and Drug Resistance Analysis of Streptococcus from Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 745-753.
[14]	XIAO Yangyang, LI Ruirui, MA Zhongchen, TANG Tian, WANG Na, CHEN Chuangfu, ZHENG Wei, WANG Yong, WANG Pengyan. Isolation,Identification and Drug Sensitivity Analysis of Mycoplasma bovis in Shihezi Region of Xinjiang [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 754-763.
[15]	MA Xiaojiao, ZHAO Yankun, SHAO Wei, WU Yating, LI Ming, LIU Huimin, MENG Lu, CHEN He. Isolation,Identification and Drug Resistance Analysis of Staphylococcus aureus in Raw Milk in Some Areas of Xinjiang [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 789-797.

Isolation and Identification of a Resistant K.peneumoniae Strain from Rabbits

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments