羊口疮病毒感染对巨噬细胞RAW264.7凋亡的影响

Abstract

Abstract: Orf was an acute skin zoonosis caused by Orf virus (ORFV), which could affect sheep, goats and humans. This research used purified ORFV (OV/HLJ/4 strains) as experimental material, the macrophage cell line RAW264.7 as host, RAW264.7 were classically activated by LPS before ORFV inoculation in vitro, the effect for macrophage apoptosis upon ORFV infection was investigated simultaneously. Comparison of morphological features of activation in the cells treated with LPS and non-LPS, these resulted in significant morphological variation each other. The apoptosis quantity of macrophage cells treated with the LPS was bigger than the non-LPS group which was less of relative changes. Western blotting showed that the apoptosis signal Caspase-3 weakened at 24 h comparing with glyceraldehyde 3-phosphate dehydrogenase (GAPDH), such a poptosis signal that was significantly weakened, related to that macrophage apoptosis level declined at that time.

Key words: Orf virus; macrophage; cell apoptosis; differentiation

CLC Number:

S482

YU Yong-zhong, LIU Jing, SUN Hu-nan, FENG Li, CUI Yu-dong. The Influence on Apoptosis of Macrophage RAW264.7 during ORFV Infection[J]. , 2013, 40(7): 25-28.

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The Influence on Apoptosis of Macrophage RAW264.7 during ORFV Infection

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