Abstract: The study was aimed to establish a method for determining Irisflorentin in Shegan Dilong granules and inspection for the stability of the preparation. RP-HPLC method was adopted and the GL Sciences Inertsil ODS-3 (150 mm×4.6 mm, 5 μm) was used. The mobile phase was acetonitrile-0.2% phosphoric acid solution (39:61), with the flow rate of 1 mL/min. The detection wavelength was at 266 nm and the column temperature was 30℃. Under the high-light exposure experiment,accelerating experiment and room temperature storage observation, the results showed that the product was basically stable. The calibration curve of Irisflorentin was linear in the range of 0.3422 to 86.60 μg/mL, r was 0.9995 (n was 10), the average recovery rate was 99.80%, RSD was 1.50% (n was 9). The method was simple, sensitive and accurate with good repeatability, which could be used for the quality control of Shegan Dilong granules and the preparation had a good stability.

Key words: RP-HPLC; Shegan Dilong granules; Irisflorentin; stability