不同浓度胰岛素样生长因子-Ⅰ对体外培养奶牛乳腺上皮细胞乳蛋白和脂肪合成相关基因表达的影响

Abstract

Abstract: The effects of different concentration (0, 1, 10 and 100 ng/mL) insulin-like growth factor-Ⅰ (IGF-Ⅰ) on the mRNA expression of genes related to milk protein and fat synthesis in bovine mammary epithelial cells cultured in vitro were evaluated by Real-time PCR. The results showed that the mRNA abundance of insulin like growth factor-Ⅰ receptor (IGFIR), insulin-like binding protein 3 (IGFBP3), α-s1-casein (CSN1S1) and κ-casein (CSN3) did not differ significantly (P>0.05) within different concentrations of IGF-Ⅰ. However, with the increasing concentrations of IGF-Ⅰ, the mRNA abundance of β-casein (CSN2), acetyl-CoA carboxylase (ACACA), fatty acid synthase (FASN) and fatty acid binding protein 3 (FABP3) increased significantly (P<0.05). These results indicated that IGF-Ⅰ, as an important cytokine, might involve in regulating the mRNA expression of genes related to milk protein and fat synthesis in bovine mammary epithelial cells cultured in vitro.

Key words: insulin like growth factor-Ⅰ; mammary epithelial cells; Real-time PCR; gene expression

CLC Number:

Q786

WANG Hao-yu, QIN Tong, HAO Hai-sheng, DU Wei-hua, ZHAO Xue-ming, ZHU Hua-bin. Effects of Different Concentration Insulin-like Growth Factor-Ⅰ on Expression of the Related Genes of Milk Protein and Fat Synthesis in Bovine Mammary Epithelial Cells Cultured in vitro[J]. , 2013, 40(7): 1-5.

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Effects of Different Concentration Insulin-like Growth Factor-Ⅰ on Expression of the Related Genes of Milk Protein and Fat Synthesis in Bovine Mammary Epithelial Cells Cultured in vitro

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