宠物犬源大肠杆菌氟苯尼考耐药基因floR的检测及药物敏感性分析

Abstract

Abstract: FloR was one of the main specific drug resistance genes to florfenicol.There was a paucity of data for the detection of floR in Escherichia coli isolated from pet dogs.The survey of floR gene by PCR and MICs of 10 common antibiotics by micro-broth dilution were performed in this study.The results indicated that among 20 Escherichia coli isolates, the resistant rate to florfenicol was 65% there were 9 and the floR-positive strains, positive rate was 45%. It could be concluded that the resistance rate of florfenicol for pet Escherichia coli isolates would appear continually increasing along with this antibiotic extensive use in animals clinical diseases.So it was very necessary to supervise florfenicol resistance among Escherichia coli isolates from pet dogs.

Key words: pet dogs; Escherichia coli; florfenicol; floR; multiple drug resistance

CLC Number:

S859.79^＋6

HE Zhi-pei, WANG Ling-fei, LIU Jian-hua, WU Hua, PAN Yu-shan, YUAN Li, HU Gong-zheng. Detection of floR Gene in Escherichia coli Isolated from Pet Dogs and Investigation of Antibiotic Susceptibility[J]. , 2013, 40(6): 211-215.

References

1 于学辉,李旭玲,杨发龙,等.鸭源致病性大肠杆菌耐氟苯尼考floR基因的克隆与序列分析[J].中国畜牧兽医,2010,37(3):84~87.
2 王显峰.犬大肠杆菌的诊治[J].中国畜牧兽医,2010,37(3):220~221.
3 刘立涛,万莉红,邵振俊,等.Ⅰ类整合子介导大肠埃希菌与肺炎克雷伯菌耐药机制的初步研究[J].中国抗生素杂志,2012,37(2):132~136.
4 师志海,陈朝喜,吴玄光,等.120株宠物源性大肠杆菌耐药性调查分析研究.中国畜牧兽医学会2008年学术年会暨第一届中国兽医临床大会.2008.
5 杜向党,牛蓓蓓,沈建忠.牛源致病性大肠杆菌耐氟苯尼考floR基因的克隆与序列分析[J].中国兽医学报,2005,25(3):247~250.
6 杜向党,李新生,张素梅,等.鸡源大肠杆菌氟苯尼考耐药基因floR的分子检测[J].河南农业大学学报,2007,41(2):188~191.
7 何柳,孙艳,雷涛,等.宠物源大肠杆菌耐药性调查[J].中国兽医杂志,2009,45(8):28~30.
8 何敏,卢胜明,李英伦.氟苯尼考耐药性研究进展[J].中国兽药杂志,2005,39(6):38~41.
9 张海旺,邓旭明,任晓慧,等.主动外排系统介导的大肠杆菌多重耐药性的确证[J].中国兽医学报,2005,25(3):173~175.
10 郑朝朝,刘聚祥,李辉,等.鸡源、猪源大肠杆菌耐氟苯尼考基因的检测与序列分析[J].华北农学报,2010a,25(5):72~75.
11 郑朝朝,刘聚祥,李辉,等.猪源大肠杆菌耐氟苯尼考基因的检测及序列分析[J].畜牧与兽医,2010b,42(3):58~60.
12 薛原,陈建飞,张秀英,等.猪源大肠杆菌中氯霉素类药物耐药基因的检测与分析[J].养猪,2011(3):71~72.
13 Axel C, Sylvie B, Geraldine F, et al. Plasmid-mediated florfenicol resistance encoded by the floR gene in Escherichia coli isolated from cattle[J]. Antimicrob Agents Chemotherapy,2000,44:2858~2860.
14 Bolton I F, Kekkey L C, Lee M D, et al. Detection of multidrug-resistance Salmonella enterica serotype typhimurium DT104 based on a gene which confers cross-resistance to florfenicol and chloramphenicol[J].J Clin Microb, 1999,37(5):1348~1351.
15 Bradford P A.Extended-spectrum beta-lactamases in the 21th century: Characterization, cpidemiology, and detection of this important resistance threat[J].Clin Microbiol Rew,2001,14:933~951.
16 Clinical and Laboratory Standards Institute. Performance standards for antimicrobial disk susceptibility tests for bacteria isolated from animals;approved standard,3rd ed[S]. CLSI,Wayne,PA,2008.
17 Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing;18th informational supplement[S].CLSI,Wayne,PA,2008.
18 Doublet B, Schwarz S,Nubbeck E,et al.Molecular analysis of chromosomally florfenicol-resistant Escherichia coli isolates from France and Germany[J].J Antimicrob Chemother,2002,49:49~54.
19 Doublet B, Schwarz S, Kehrenberg C.Florfenicol resistance gene floR is part of a novel transposon[J].Antimicrob Agents Chemother,2005,49(5):2106~2108.

[1]	WU Liting, LU Rui, LIU Banhong, BAO Hongduo, WANG Yongjuan, ZHOU Yan, WANG Ran, ZHANG Hui. Combination of Clostridium perfringens Phage and Lactobacillus Improves Growth Performance and Regulates Intestinal Flora Function of Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2321-2332.
[2]	ZHANG Huaqiang, WANG Pinsheng, LI Xiao, WEI Panpeng, YAN Lingmin, JIANG Bingbing, WANG Zhaogui, WANG Xuefang, ZENG Huanqing, FAN Yi, GAO Zhenglong, WANG Xuebing. Effects of Fermented Hybrid Broussonetia papyrifera on Growth Performance, Meat Quality,Digestive Enzyme Activity and Intestinal Flora Structure of Hu Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1876-1887.
[3]	CUI Shuang, Wurlig, Alder, XIAO Min, WANG Jianqiu, GONG Jian. Effects of Yeast Selenium Supplementation on Gastrointestinal Microflora in Sheep Under Grazing Conditions [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 952-965.
[4]	SUN Yuelong, ZHANG Mengjie, DOU Jiahong, WANG Xiaoying, DAI Xiaofeng, LI Xiumei. Effects of Astragalus membranaceus (Fisch.) Bunge.on Piglets Flora Imbalance Diarrhea Based on Network Pharmacology and Molecular Docking [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3200-3211.
[5]	SHI Jiancun, LI Jikai, JIA Lin, LYU Jiancun, SHANG Zihan, ZHANG Tie, WANG Chunguang. Effects of Fermented Traditional Chinese Medicine on the Growth Performance, Antibody Level and Intestinal Flora of Laying Hens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2145-2155.
[6]	HAO Wenjun, XIA Hongze, JIAO Yang, CUI Zhanhong, LIU Shujie, ZHOU Lei, ZHOU Yuqing. Effects of Different Roughage Grading Index and Carbohydrate Balance Index Combinations Diets on Bacterial Flora in the Rumen and Rectum of Yaks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1715-1726.
[7]	BAI Peitian, KONG Jiamei, PEI Ting, CHENG Zhixue, REN Yuhong. Effects of Compound Microecological Preparation on Growth Performance, Immune Function and Caecal Flora Structure of Weaned Piglets [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 942-952.
[8]	ZHANG Lihuan, JIA Hao, ZHANG Ruonan, WANG Yanfei, LIU Xuan. Effects of Compound Probiotics on the Intestinal Microflora of Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 488-500.
[9]	WANG Zhihua, JIA Junpeng, RADEBE Stoffel Matjeke, YU Qinghua. Study on the Growth Performance and Intestinal Flora of Beagle Puppies Regulated by Probiotics [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 510-520.
[10]	SHAN Chunqiao, ZHAI Hongxu, LI Juan, WANG Yan, YU Hongmin, WU Yiqi, LIU En, LIU Yan. Effects of Clostridium butyricum on Intestinal Short-chain Fatty Acid Content and Microflora Diversity of Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4239-4251.
[11]	ZHONG Chonghua, GUI Fuxing, CAO Liting, GUO Rongfu. Research Advances of Hermetia illucens on Intestinal Health and Microflora in Pigs and Poultry [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3829-3837.
[12]	ZHANG Yuying, LIU Shujie, FENG Yuzhe, ZHANG Xiaowei, CUI Zhanhong. Bacterial Flora Composition of Normal and Diarrhea Yak Calves Based on 16S rRNA Sequencing [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3293-3302.
[13]	TAO Zhiyun, ZHU Chunhong, SHI Zuhao, XU Wenjuan, ZHANG Shuangjie, SONG Weitao, LIU Hongxiang, LI Huifang. Effect of Riemerella anatipestifer Infection on the Microflora of Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2129-2139.
[14]	CAO Mixia, GUAN Jingyuan, YANG Jian, WANG Xinrui, WANG Lingli, BIN Xiujuan, WANG Kefei, HUANG Haizi, HU Tingjun. Effect of Passiflora edulis Polysaccharide Extract on Oxidative Stress of Porcine Circovirus Type 2 Infected RAW264.7 Cells [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2238-2246.
[15]	GENG Yumeng, LI Shuying, LU Chunyu, CUI Yuqing, SHI Wanyu, BAO Yongzhan. Study on the Molecular Mechanism of Salvia miltiorrhiza Polysaccharide in Inhibiting Florfenicol-induced Liver Injury in Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1849-1858.

Detection of floR Gene in Escherichia coli Isolated from Pet Dogs and Investigation of Antibiotic Susceptibility

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments