羊毛尾线虫线粒体nad4基因的序列变异分析

Abstract

Abstract: The objectives of the present study were to analyze sequence variation in the NADH dehydrogenase subunit 4 (nad4) gene among Trichuris ovis isolates from Guangdong province in China, and to study its phylogenetic relationships with other whipworms using the nad4 gene sequences. The partial nad4 (pnad4) were amplified from individual T.ovis samples, and pnad4 sequences were aligned using the ClustalX 1.81. Neighbor-joining (NJ) trees were constructed using the software Mega version 5.0. The lengths of all pnad4 sequences were 827 bp. Phylogenetic analyses showed that all T.ovis isolates were clustered in the same clade.Sequence variations in pnad4 sequences within T.ovis were 0 to 1.5%, which was significantly lower than inter-species differences (37.8% to 45.6%). It was concluded that pnad4 sequence could be used as a genetic marker for population genetic studies of T.ovis, as well as for the differentiation and identification of different whipworms. The results of this study provided foundation for further studies on molecular genetics and diagnostics of T.ovis.

Key words: Trichuris ovis; mitochondrial DNA; NADH dehydrogenase subunit 4 gene; phylogenetic relationship

CLC Number:

WANG Yan, YANG Yan-chuan, ZHANG Yue-wen, LI Jia-yuan, ZHU Xing-quan, LIU Guo-hua. Analysis of Sequence Variation in Mitochondrial nad4 Gene of Trichuris ovis Isolates[J]. , 2013, 40(4): 81-84.

References

1 李淼,蔡丝丝,姚明锐,等. 湖南省鸡蛔虫线粒体nad4基因序列的测定及分析[J].现代农业科技,2012,4:321~325.
2 王燕,刘国华,李佳缘,等.中华双腔吸虫线粒体nad4基因的克隆及序列分析[J].中国畜牧兽医,2012,39(5):118~120.
3 刘伟, 刘毅, 林瑞庆,等. 日本血吸虫线粒体nad4基因部分序列的多态性研究[J].中国寄生虫学与寄生虫病杂志,2008,26(4):152~153.
4 伍慧兰. 猬裂头蚴线粒体nad4基因的克隆及序列分析[J].动物医学进展,2010,31(12):61~63.
5 Abebe R, Gebreyohannes M, Mekuria S, et al. Gastrointestinal nematode infections in small ruminants under the traditional husbandry system during the dry season in Southern Ethiopia[J]. Trop Anim Health Prod,2010,42(6):1111~1117.
6 Bethony J, Brooker S, Albonico M, et al. Soil-transmitted helminth infections: Ascariasis,trichuriasis,and hookworm[J]. Lancet,2006,367(9521):1521~1532.
7 Bethony J M, Cole R N, Guo X, et al. Vaccines to combat the neglected tropical diseases[J]. Immunol Rev, 2011,239(1):237~270.
8 Blouin M S. Molecular prospecting for cryptic species of nematodes: Mitochondrial DNA versus internal transcribed spacer[J]. Int J Parasitol, 2002,32(5):527~531.
9 Callejon R, De R M, Ariza C, et al. Cytochrome oxidase subunit 1 and mitochondrial 16S rDNA sequences of Trichuris skrjabini (Tricocephalida: Trichuridae)[J]. Parasitol Res,2009,104(3):715~716.
10 Crompton D W. How much human helminthias is there in the world?[J]. J Parasitol,1999,85(3):397~403.
11 Cutillas C, De Rojas M, Ariza C, et al. Molecular identification of Trichuris vulpis and Trichuris suis isolated from different hosts[J]. Parasitol Res,2007,100(2):383~389.
12 Knight R A. Redescription of Trichuris discolor (von Linstow, 1906) and T.skrjabini (Baskakov,1924) from domestic ruminants in the United States and comparisons with T.ovis (Abildgaard,1795)[J]. J Parasitol,1971,57(2):302~310.
13 Liu G H, Gasser R B, Su A, et al. Clear genetic distinctiveness between human- and pig-derived Trichuris based on analyses of mitochondrial datasets[J].PLoS Negl Trop Dis,2012,6(2):e1539.
14 McManus D P, Bowles J.Molecular genetics approaches to parasite identification: Their value in diagnostic parasitology and systematics[J]. Int J Parasitol,1996,26(7):687~704.
15 Roepstorff A, Mejer H, Nejsum P, et al. Helminth parasites in pigs: New challenges in pig production and current research highlights[J]. Vet Parasitol, 2011,180(1~2):72~81.
16 Spakulová M. Discriminant analysis as a method for the numerical evaluation of taxonomic characters in male trichurid nematodes[J]. Syst Parasitol,1993,29(2):113~119.

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Analysis of Sequence Variation in Mitochondrial nad4 Gene of Trichuris ovis Isolates

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