4种佐剂对绵羊肺炎支原体的免疫辅佐效力研究

Abstract

Abstract: Different types of adjuvants are different on the alloantigen immune adjuvant effect. To screen the ideal adjuvant of the inactivated vaccine of Mycoplasma ovipneumoniae (MO), the inactivated vaccines using 4 different adjuvants were respectively immunized adult rabbits and the determination of serum antibody titers were detected using the indirect hemagglutination kit after immunization from 1 to 9 weeks. The results showed that the 4 different vaccines could quickly produce antibodies after immunization. Furthermore, the rabbits antibodies using Buonavoglial's and ISA-760 adjuvant vaccines peaked at 3 weeks after vaccination ((5.67±0.70) log2,6.00 log2), and maintained the peak until in 9 weeks (6.00 log2,(6.00±1.00) log2). In addition to vaccines with MO ISA-206 and white oil adjuvant, the rabbit antibodies peaked in 4 weeks after immunization ((4.33±0.57) log2,5.00 log2) and decreased a titer until 9 weeks. According to the statistical analysis, the results showed that the immune enhancement effect of ISA-760 and Buonavoglial's adjuvant for the MO was significantly better than that of ISA-206 and white oil adjuvant (P<0.05). Therefore, the above results indicated that ISA-760 and Buonavoglial's were an ideal candidate for MO inactivated vaccine adjuvant and provided a scientific basis for studing the efficient of the inactivated vaccine for MO.

Key words: Mycoplasma ovipneumoniae; inactivated vaccine; immune effectiveness; adjuvant

CLC Number:

S858.28

FEI Lei, YUE Hua, TANG Cheng. Study on the Four Adjuvant for Inactivated Vaccine of Mycoplasma ovipneumoniae Immune Effectiveness[J]. , 2013, 40(3): 220-223.

References

1 王继红,邵国青,何家惠,等.猪肺炎霉形体168弱毒株的回归试验[J].中国兽医科技,2005,31(3):18～19.
2 何存利,鲍嘉铭,郑丽侠.绵羊肺炎霉形体疫苗制备及免疫效果检测[J].甘肃畜牧兽医,2005,35(3):18～20.
3 郭军华,胡松华,宋培武.瑞倍适(猪肺炎支原体灭活疫苗)田间免疫效果观察[J].畜牧与兽医,2009,9:106.
4 储岳峰,赵萍,高鹏程,等.绵羊和山羊霉形体研究概述[J].畜牧兽医科技信息,2008(6):8～11.
5 储岳峰.中国山羊(接触)传染性胸膜肺炎病原学、流行病学研究及灭活疫苗的研制.兰州:中国农业科学院,2011.
6 韩笑,岳华,王远微,等. 绵羊肺炎支原体灭活疫苗的研制[J].中国畜牧兽医, 2011, 38(11): 181～184.
7 樊庆德,徐春光,高明华.绵羊三种支原体免疫预防研究进展[J].动物医学进展,2011,32(6):156～159.
8 Besser T E,Cassirer E F,Potter K A,et al.Association of Mycoplasma ovipneumoniae infection with population-limiting respiratory disease in free-ranging Rocky Mountain bighorn sheep (Ovis Canadensis canadensis)[J].J Clin Microbiol,2008,46:423～430.
9 Besser T E,Cassirer E F,Yamada C, et al. Survival of bighorn sheep (Ovis canadensis) commingled with domestic sheep (Ovis aries) in the absence of Mycoplasma ovipneumoniae[J].J Wildl Dis,2012,48(1):168～172.
10 Cupla R K,Relyve E H,Lindblad E B,et al.Adjuvant balance between luxieity and adjuvanlicity[J].Vaccine,1993,11:293～306.
11 Domenico B,Grazia G,Vincenzo Q,et al.An oil-emulsion vaccine induces full-protection against Mycoplasma agalactiae infection in sheep[J].New Microbiologica,2008,31:117～123.
12 Loens K,Mackay W G,Scott C,et al.Amulticenter pilot external quality asscssment programme to assess the quality of molecular detection of Chlamydophila pneumonia and Mycoplasma pneumonia[J].J Microbiol Meth,2010,82(2):131～135.
13 Mackay J M K,Nisbet D I,Foggie A.Isolation of pleuropneumonia-like organisms (genus Mycoplasma) from cases of sheep pulmonary adenomatosis[J].Vet Rce,1963,75:550～551.
14 Parham K,Churchward C P,McAuliffe L,et al.A high level of strain variation within Mycoplasma ovipneumoniae population of the UK has implications for disease diagnosis and management[J].Vet Microbiol,2006,118:83～90.
15 Patel H,Mackintosh D,Ayling R D,et al.A novel medium devoid of ruminant peptone for high yield growth of Mycoplasma ovipneumoniae[J].Vet Microbiol,2008,127(3～4):309～314.
16 Thiaucourt F,Bölske G.Contagious caprine pleuropneumonia and other pulmonary mycoplasmoses of sheep and goats[J].Rev Sci Tech,1996,15(4):1397～1414.

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Study on the Four Adjuvant for Inactivated Vaccine of Mycoplasma ovipneumoniae Immune Effectiveness

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