猪繁殖与呼吸综合征病毒山西分离株ORF5基因的序列分析

Abstract

Abstract: Through RT-PCR amplification of tissue or blood sample from Shanxi province, the 5 ORF5 gene nucleotide sequences were obtained. The obtained sequences and the deduced amino acid were analyzed and compared with the other published PRRSV strains. The nucleotide homology were 97.7% to 98.5% among 5 ORF5,comparing with LV, the strains isolated from China in 2006 to 2007, the strains isolated before 2006, Resp PRRS MLV and MLV RespPRRS/Repro, the nucleotide homology were 63.5% to 64.0%, 88.7% to 89.2%, 97.7% to 99.3%, 88.1% to 96.7%, 88.6% to 89.1%.The aniniacid homology were 56.1% to 56.6%,87.8% to 88.8%,96.6% to 98.5%,85.9% to 93.2%,86.8% to 87.9%, respectively. The results showed that the prevalent PRRSV strains in Shanxi belong to North American genotype which had high homology and correlative genetic relationship.

Key words: PRRSV; ORF5 gene; variation analysis

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LIU Wen-jun, YAO Jing-ming, WU Xin, WANG Juan-ping, MENG Fan, HAN Yi-chao, FAN Zhen-hua, MI Rui-juan, LUO Tian-tian, WEN Yong-liang. Sequence Analysis of ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus Isolated from Shanxi Area[J]. , 2013, 40(2): 10-13.

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Sequence Analysis of ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus Isolated from Shanxi Area

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