China Animal Husbandry & Veterinary Medicine ›› 2013, Vol. 40 ›› Issue (2): 10-13.

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Sequence Analysis of ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus Isolated from Shanxi Area

LIU Wen-jun1, YAO Jing-ming1, WU Xin1, WANG Juan-ping1, MENG Fan1, HAN Yi-chao1, FAN Zhen-hua1, MI Rui-juan1, LUO Tian-tian2, WEN Yong-liang1   

  1. 1. Institution of Animal Husbandry and Veterinary, Shanxi Academy of Agricultural Sciences, Taiyuan 030032, China;
    2. Shanxi Provincial Center for Animal Disease Prevention and Control, Taiyuan 030024, China
  • Received:2012-09-06 Online:2013-02-20 Published:2013-02-06

Abstract: Through RT-PCR amplification of tissue or blood sample from Shanxi province, the 5 ORF5 gene nucleotide sequences were obtained. The obtained sequences and the deduced amino acid were analyzed and compared with the other published PRRSV strains. The nucleotide homology were 97.7% to 98.5% among 5 ORF5,comparing with LV, the strains isolated from China in 2006 to 2007, the strains isolated before 2006, Resp PRRS MLV and MLV RespPRRS/Repro, the nucleotide homology were 63.5% to 64.0%, 88.7% to 89.2%, 97.7% to 99.3%, 88.1% to 96.7%, 88.6% to 89.1%.The aniniacid homology were 56.1% to 56.6%,87.8% to 88.8%,96.6% to 98.5%,85.9% to 93.2%,86.8% to 87.9%, respectively. The results showed that the prevalent PRRSV strains in Shanxi belong to North American genotype which had high homology and correlative genetic relationship.

Key words: PRRSV; ORF5 gene; variation analysis

CLC Number: