饲料及其原料中沙门氏菌的分离鉴定和耐药分析

Abstract

Abstract: To establish the theoretical basis for the prevention and control of Salmonella spreading, the experiment was to analyze the contamination and drug resistance of Salmonella in feed and its original materials. 707 samples were detected and Salmonella were isolated and identified in accordance with the methods GB/T 13091－2002, the serotype was classified in accordance with GB 4789.4－2010, and Kirby-Bauer disc diffusion method was used to detect the resistance of strains. The results showed that fifty-six (7.9%) strains were demonstated as positive, 52 of which were from the materials of animal origin. Four serotypes were found, including Agona, AgonaⅡ, Bournemouth, Mokola. By drug susceptibility test, the antibiotic rate of Salmonella in feed was much lower than those originated from human and animals, the rest of strains due to the categeries limitaion of serum, the serotype only classified into the group, failed to further classification. Drug sensitive test showed that there were still one strain of 13 multidrug-resistant, two strains of 4 multidrug-resistant, twelve strains of 2 multidrug-resistant and nine strains of 3 multidrug-resistant with different resistance spectrum. Although the test demonstrated relatively low percentage and level of Salmonella in the feed, there still had a few individual strains with serious drug resistance, so it was necessary to watch the application of antibiotics in animal husbandry strictly.

Key words: Salmonella; serotype; drug-resistance

CLC Number:

S852.61

SHE Rong, LIU Yao-min, DU Xue-li, YAN Qi-gui. Identification and Antibiotic Analysis of Salmonella Isolated from Feed and its Original Materials[J]. , 2013, 40(12): 204-207.

References

1 中华人民共和国卫生部.GB 4789.4－2010食品安全国家标标准食品微生物学检验沙门氏菌检验[S].北京:中国标准出版社,2010.
2 中华人民共和国国家质量监督检验检疫总局.GB/T 13091－2002 饲料中沙门氏菌检测[S].北京:中国标准出版社,2002.
3 王红琳,杨峻,邵华斌,等. 鹌鹑致病性大肠杆菌与沙门氏菌混合感染的病原分离鉴定与药敏试验[J].畜牧与饲料科学,2010,31(1):156~158.
4 王兵. 沙门氏菌引起的食物中毒[J].当代医学,2012,18(23):160.
5 王傲雪,张凌云,张桉潮,等. 肉鸡沙门氏菌的分离鉴定及药敏试验[J].中国畜牧兽医,2013,40(7):167~171.
6 王斌,孙自镛,申正义,等. 武汉地区粪便中非伤寒沙门氏菌的耐药性与流行病学调查[J].中国人兽共患病学报,2006(1): 92~93.
7 朱小妮,沈红良,肖红,等. API鉴定系统在临床微生物检验中的应用[J].中国医师杂志,2004(S1):295~296.
8 朱琼媛,朱姝媛,张彦,等. 74例甲型副伤寒沙门菌药敏结果及临床分析[J].西南国防医药,2008(2):244~245.
9 朱超,徐学斌. 沙门菌属血清型诊断[M].上海:同济大学出版社,2009.
10 张燕,朱超. 我国沙门氏菌病和菌型分布概况[J].现代预防医学,2002(3):400~401.
11 李卫东,刘晓娟,郑秀清,等. 一起由沙门氏菌引起食物中毒的调查分析[J].医学动物防制,2011,27(1):56~58.
12 李庆德,原志伟,沈巍. 沙门氏菌的危害及其快速检测方法的研究进展[J].湖北畜牧兽医,2010,165(1):10~12.
13 陈春梅,王斌,陈宗辉,等. 4种检测技术对冷冻食品沙门氏菌检测效果的比较[J].检验检疫科学,2006,16:68~71.
14 段永翔. API鉴定系统及其在细菌学检验中的应用[J].现代预防医学,2004(5):729~731.
15 顾雪影. 62株伤寒、甲型副伤寒沙门氏菌的鉴定及药敏试验[J].预防医学文献信息,2001(3):268~271.
16 Dione M M,Geerts S,Antonio M. Characterisation of novel strains of multiply antibiotic-resistant Salmonella recovered from poultry in Southern Senegal[J].Journal of Infection in Developing Countries,2012,6(5): 436~442.
17 Louden B C,Haarmann D,Han J,et al. Characterization of antimicrobial resistance in Salmonella enterica serovar typhimurium isolates from food animals in the U.S.[J].Food Research International,2012,45(2):968~972.
18 Maddocks S,Olma T,Chen S. Comparison of CHRO Magar Salmonella medium and xylose-lysine-desoxycholate and Salmonella-Shigella agars for isolation of Salmonella strains from stool samples[J].Journal of Clinical Microbiology,2002,40(8):2999~3003.
19 Nayak R,Stewart T,Wang R F,et al. Genetic diversity and virulence gene determinants of antibiotic-resistant Salmonella isolated from preharvest turkey production sources[J].International Journal of Food Microbiology,2004,91(1):51~62.
20 Pulsrikarn C,Chaichana P,Pornruangwong S,et al. Serotype,antimicrobial susceptibility,and genotype of Salmonella isolates from swine and pork in Sa Kaew province,Thailand[J].Thai Journal of Veterinary Medicine,2012,42(1): 21~27.
21 Van T T H,Nguyen H N K,Smooker P M,et al. The antibiotic resistance characteristics of non-typhoidal Salmonella enterica isolated from food-producing animals,retail meat and humans in South East Asia[J].International Journal of Food Microbiology,2012,154(3):98~106.
22 Wang L,Mustapha A. EMA-Real-time PCR as a reliable method for detection of viable Salmonella in chicken and eggs[J].Journal of Food Science,2010,75(3):M134~M139.

[1]	CAO Qi, JIN Qing, YUAN Yi, FENG Yixue, KONG Danni, CHEN Xianghong, WU Bin, SONG Wenbo, TANG Xibiao. Epidemiological Investigation of Common Respiratory Bacterial Pathogens in Large-scale Pig Farms [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2439-2449.
[2]	WANG Xueping, ZHANG Xiaojun, LI Xiaoyue, WANG Guodong, ZHANG Fuliang, SONG Yuwei, ZHANG Mingliang, MA Lei. Prokaryotic Expression of Hexon Protein of Highly Pathogenic Fowl Adenovirus Serotype 4 and Preparation of Polyclonal Antibodies [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2053-2060.
[3]	CHEN Dong, ZHANG Yanhao, ZHANG Yang, LI Sen, ZHOU Zutao, LI Zili. Isolation,Identification and Drug Sensitivity of 3 Strains of Riemerella anatipestifer [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1250-1258.
[4]	TIAN Erjie, GU Zhen, WANG Zihao, LIU Fangzheng, LIU Kesen, ZHANG Xuefei, ZHOU Bianhua. Study on the Drug-resistance Elimination of Scutellaria baicalensis on Apramycin-resistant Escherichia coli [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1279-1286.
[5]	YUAN Cheng, CHEN Huaijun, YUAN Sheng, WEN Huamao, CHEN Zhiyi, DONG Hongyan, FENG Qi, GUO Changming. Isolation, Identification and Study on Virulence Gene and Drug Resistance of Streptococcus agalactiae from Tilapia in Zhangzhou City, Fujian Province [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3655-3664.
[6]	WANG Zhifang, XU Yindi, ZHU Wenhao, ZHANG Qingxian, JIAO Wenqiang, LI Haili, WANG Keling. Analysis of Virulence and Drug Resistance of Haemophilus parasuis Serotype 7 Strains [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3245-3255.
[7]	LI Qianqian, LONG Yunzhi, LIANG Gong, JIN Qing, LIU Jinjin, YANG Liu, HUANG Ying, YU Daobing, SONG Wenbo, HUANG Chao, TANG Xibiao. Screening of Vaccine Candidate Strains of Streptococcus suis Serotype 3 and Evaluation of the Immune Effect of the Bivalent Inactivated Vaccine Streptococcus suis Serotype 3+9 on Mice [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2307-2317.
[8]	WANG Simin, YANG Jiangfeng, ZHAO Xiaokun, ZHANG Lei, GUO Jingjing, LAN Jinping, YU Xiaojie, WANG Jing. Isolation, Identification and Analysis of Pathogenicity and Drug Resistance of Salmonella from Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2385-2396.
[9]	DONG Hongyan, SONG Haigang, ZHU Shanyuan, YU Shengqing, XIE Jun, HONG Shuangpeng, GUO Changming, XU Hai, GAO Yuexiu. Isolation,Identification and Pathogenicity Analysis of Escherichia coli from Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1977-1984.
[10]	WNAG Tongzhao, ZHANG Fuxian, LEI Liancheng. Isolation, Identification and Drug Resistance Analysis of a Strain of Haemophilus parasuis Serotype 4 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1096-1105.
[11]	SHANG Yu, LI Lintao, LI Li, XU Yingying, FENG Helong, WANG Hongcai, ZHANG Rongrong, ZENG Zhe, WANG Honglin, LUO Qingping, SHAO Huabin, WEN Guoyuan. Construction of Recombinant Heat-resistant Newcastle Disease Virus Expressing Fiber2 Protein of Fowl Adenovirus Serotype 4 and Evaluation of Its Immune Effect [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 650-659.
[12]	XU Ting, XIONG Ting, LI Linyu, LIU Yufu, WEN Lianghai, XIE Wenting, YANG Zekun, CHEN Ruiai. Construction and Identification of Recombinant Replication-deficient HAdV-5 Expressing VP2 Protein of Infectious Bursal Disease Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4524-4534.
[13]	ZHANG Haolei, SALANG Wenzhu, HUANG Zhihong, WEN Yongping, ZHANG Huanrong. Isolation, Identification, Drug Resistance and Pathogenicity Analysis of Salmonella from Duck [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4887-4897.
[14]	CHEN Huaijun, GUO Changming, ZHU Shanyuan, WU Zhi, YUAN Sheng, WANG Yongjuan, YUAN Cheng, PAN Yong, WANG Xiaoye. Isolation,Identification and Biological Characteristics of Streptococcus agalactiae from Tilapia in Hainan [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 3058-3068.
[15]	GUO Changming, CHEN Huaijun, YUAN Cheng, LUO Fuguang, HUANG Desheng, JIA Guohua, YUAN Sheng, ZHU Shanyuan. Isolation,Identification,Virulence Genes and Antibiotic Resistance Analysis of Streptococcus agalactiae from Tilapia in Guangxi [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2607-2616.

Identification and Antibiotic Analysis of Salmonella Isolated from Feed and its Original Materials

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments