不同血清型猪链球菌对新西兰兔的致病性研究

doi:10.16431/j.cnki.1671-7236.2024.06.031

Abstract

Abstract: 【Objective】 This study was aimed to investigate the distribution pattern and differences in pathogenicity of different serotypes of Streptococcus suis (SS) in New Zealand rabbits,so as to provide reference for the prevention and control of porcine streptococcal disease.【Method】 In this study,New Zealand rabbits were infected with SS1 ZY194,SS2 QH and SS7 ZY188 strains,respectively,and the clinical symptoms were observed,the pathological tissue changes were analyzed by HE staining.The organ bacterial load was detected using Real-time quantitative PCR,the changes in biochemical indexes of serum and liver were detected by kit,and the expression of related inflammatory factors were detected by ELISA.【Result】 The clinical symptoms and the degree of pathological changes in organs of New Zealand rabbits in SS2 group were the most obvious among 3 groups.All the organs of New Zealand rabbits in experimental groups were bacterial-carrying,and the bacterial load of organs in SS2 group was higher than that in SS1 and SS7 groups,and spleen was the organ with the highest bacterial load.The contents of glucose (GLU),triglyceride (TG),total cholesterol (TCHO),low density lipoprotein (LDL) and uric acid (UA) of New Zealand rabbits in experimental groups were extremely significantly or significantly higher than those in control group (P＜0.01 or P＜0.05),and the content of high density lipoprotein (HDL) of New Zealand rabbits in SS2 and SS7 groups were extremely significantly lower than that in control group (P＜0.01).The contents of glutathione peroxidase (GSH-Px),superoxide dismutase (SOD) and lipase (LPS),and the expression of inflammatory factors IL-6,IL-1β and TNF-α of New Zealand rabbits in experimental groups were extremely significantly or significantly higher than those in control group (P＜0.01 or P＜0.05),and the levels of each index in SS2 group were the highest.【Conclusion】 The different serotypes of Streptococcus suis could cause disease in New Zealand rabbits,among which the SS2 QH strain was the most pathogenic.The results provided a reference for the pathogenesis research on porcine streptococcal disease and a theoretical basis for clinical control.

Key words: Streptococcus suis; serotypes; New Zealand rabbits; pathogenicity

CLC Number:

S816.7

ZHANG Shuzhi, LIU Fei, WANG Zhenyong, CHEN Zhi, ZHANG Lin, SUN Wenbo, ZHANG Yuyu, DING Luogang, LI Jianda, REN Sufang, YU Jiang, WU Jiaqiang. Study on the Pathogenicity of Different Serotypes of Streptococcus suis to New Zealand Rabbits[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(6): 2566-2575.

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Study on the Pathogenicity of Different Serotypes of Streptococcus suis to New Zealand Rabbits

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