基于UniGene寻找山羊新的miRNA及其试验验证

Abstract

Abstract: Using goat UniGene to identify miRNA and RT-PCR was used to validate the predicted miRNA, and then Real-time PCR was performed to detect the miRNA expression during one year in goat skin and muscle. Results revealed that 5 new miRNA were identified and all of these miRNA were expressed in goat skin and muscle, in which a miRNA cluster was discovered which was composed of chi-miR-421*,chi-miR-421 and chi-miR-374b. miRNA expression results indicated that chi-miR-1839,chi-miR-374b and chi-miR-2284n in skin gave a highest expression level on February, chi-miR-421 and chi-miR-421*expression increased remarkably on October. The identification of 5 new miRNA in goat(chi-miR-2284n, chi-miR-421*, chi-miR-421, chi-miR-1839 and chi-miR-374b,accession number:JQ002550-JQ002554) was a useful complement for goat miRNA database.

Key words: UniGene; miRNA; goat; skin; muscle

CLC Number:

SU Rui, LV Xiao-man, ZHAO De-chao, HAN Zhi-ling, FU Shao-yin, MENG Li-yun, ZHANG Wen-guang, LI Jin-quan. Identification and Experimental Verification of Novel miRNA on UniGene in Goat[J]. , 2013, 40(11): 7-11.

References

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Identification and Experimental Verification of Novel miRNA on UniGene in Goat

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