鸡胚肠上皮细胞Toll样受体的表达谱研究

Abstract

Abstract: Chicken epithelial cells (IECs) from eighteen day-old chicken embryos were separated by the digestion of thermolysin. The chicken epithelial cells were identified by morphological observation and immunocytochemical method. TLR mRNA expression was detected with reversing transcription polymerase chain reaction (RT-PCR). TLR receptor expression in the intestinal epithelial cells of different varieties of chick were compared. The results showed that the epithelial cells of chicken embryo grew well. 90% for the purification of the IEC was obtained by removing single cells by low-speed centrifuge and differential velocity adherent. The result of identification by detecting the cyto-keratin was positive. IECs all expressed of 10 kinds of TLR mRNA with different intensity. There was no significant difference in TLR receptor expression in the intestinal epithelial cells of different varieties of chick.

Key words: chicken embryo intestinal epithelial cells; Toll-like receptor; expression profile

CLC Number:

S831

TANG Meng-jun, GAO Yu-shi, ZHANG Xiao-yan, LU Jun-xian, PU Jun-hua, GU Rong. Study on Toll-like Receptors Expression Profile in Chicken Embryo Intestinal Epithelial Cells[J]. , 2013, 40(11): 46-50.

References

1 王晓亮,顾宪红,郑冬梅,等.鸡小肠上皮细胞的分离及体外培养研究进展[J].中国畜牧兽医,2010,37(9):34~38.
2 张道杰,蒋建新,陈永华,等.用嗜热菌蛋白酶进行人肠上皮细胞分离培养[J].第三军医大学学报,2004,26(11):1016~1018.
3 Abreu M T,Fukata M,Arditi M. TLR signaling in the gut in health and disease[J].J Immunol,2007,19(2):106~115.
4 Aldhous M C,Shamkov A N,Bode J,et al. Characterization of conditions for the primary culture of human small intestinal epithelial cells[J]. Clin Exp Immunol,2001,125(1):32~40.
5 Bouhet S,Hourcade E,Loiseau N,et al. The mycotoxin fumonisin B₁ alters the proliferation and the barrier function of porcine intestinal epithelial cells[J]. Toxicol Sci,2004,77(1):165~170.
6 Greenman H S,Rutten M J,Fowler W M,et al. Herbicide/Pesticide effects on intestinal epithelial growth[J]. Environ Res,1997,75:85~93.
7 He Y P,Chu S W,Walker W A. Nucleotide supplements later proliferation and differentiation of cultures human (Caco-2) and rat (IEC-6) intestinal epithelia cells[J]. J Nutr,1993,123:1017~1027.
8 Iqbal M,Philbin V J,Smith A L. Expression patterns of chicken Toll-like receptor mRNA in tissues,immune cell subsets and cell lines[J]. Vet Immunol Immunopathol,2005,104(1~2):117~127.
9 Kang S S,Kaul L S,Gaspari A A. Toll-like receptors applications to dermatologic disease[J]. J Am Acad Dermatol,2006,54(6):951~983.
10 Kedinger M,Assmann P,Alexandre E,et al. Importance of a fibroblastic support of in vitro differentiation of intestinal endodermal cells and for their response to glucorticoids[J]. Cell Differ,1987,20(3):171~182.
11 Ortega-Cava C F,Ishihara S,Rumi M A,et al. Strategic compartment alization of Toll-like receptor 4 in the mouse gut[J]. J Immunol,2003,170(8):3977~3985.
12 Oswald I P. Role of intestinal epithelial cells in the innate immune defence of the pig intestine[J]. Vet Res,2006,37(3):359~368.
13 Sanderson I R,He Y P. Nucleotide uptake and metabolism by intestinal epithelial cells[J].J Nutr,1994,124:131~137.
14 Temperley N D,Berlin S,Paton I R,et al. Evolution of the chicken Toll-like receptor gene family: A story of gene gain and gene loss[J].BMC Genomics,2008,9:62.
15 Uehara A,Fujimoto Y,Fukase K,et al. Various human epithelial cells express functional Toll-like receptors,NOD1 and NOD2 to produce anti-microbial peptides,but not proinflammatory cytokines[J]. Mol Immunol,2007,44(12):3100~3111.

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Study on Toll-like Receptors Expression Profile in Chicken Embryo Intestinal Epithelial Cells

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