布莱凯特黑牛心脏脂肪酸结合蛋白基因单核苷酸多态性的研究

Abstract

Abstract: The single nucleotide polymorphisms(SNPs) in exon 2 and part of intron 2 of H-FABP gene in 96 individuals from Black cattle were detected by PCR-SSCP methods and sequencing analysis technique. The results showed that there were two SNPs in intron 2 at primer 2 and primer 4, no variance at primer 1 and primer 3. Three genotypes (AA, AB, BB) determined by SNP at 323 (G→A) were detected using primer 2.Their genotype frequencies were 0.448, 0.146, 0.406, which were no in Hardy-Weinberg equilibrium with chi-aquare analysis. Statistical analysis revealed an intermediate polymorphism information content (PIC) in Black cattle. Three genotypes (HH, Hh, hh) determined by SNP at 872 (C→G) were detected using primer 4.Their genotype frequencies were 0.104, 0.417, 0.479, which were in Hardy-Weinberg equilibrium with chi-aquare analysis. Statistical analysis revealed an intermediate polymorphism information content (PIC) in Black cattle.

Key words: Black cattle; H-FABP gene; SNP

CLC Number:

Q349⁺.53

ZHAO Shi-quan, DONG Ya-juan, BAI Xue-jin, NIU Zhao-shan, YANG Li, ZHANG Xin-hao. Study on Single Nucleotide Polymorphisms of H-FABP Gene in Black Cattle[J]. , 2013, 40(11): 42-45.

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Study on Single Nucleotide Polymorphisms of H-FABP Gene in Black Cattle

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