地方土猪源副猪嗜血杆菌16S rRNA基因的分子鉴定及遗传进化分析

Abstract

Abstract: To study the molecular genetic evolution characteristics of Haemophilus parasuis(Hps), PCR was used to determine the 16S rRNA gene of the strain XY0501 isolated from local pigs in Henan province, and genetic evolution analysis was conducted. The results showed that the amplication of 16S rRNA of the isolate XY0501 was 822 bp, the nucleotide sequence similarity among the reference strains was 97.1% to 99.3%, and 99.3% with the strain serotype 5. The phylogenetic tree based on 16S rRNA revealed that the Hps isolate XY0501 from local pigs belonged to the same branch with reference strain serotype 5. The results identified that the Hps infection could cause severe clinical symptoms in pigs, but infection source need to be further investigation,in addition, 16S rRNA of Hps of different serotypes was conserved and no species difference.

Key words: Haemophilus parasuis; strain from local pig; PCR; 16S rRNA; molecular genetic evolution

CLC Number:

S813.3

CHANG Hong-tao, LIU Hui-min, DU Ji-mei, CHEN Lu, ZHAO Jun, WANG Xin-wei, YAO Hui-xia, YANG Xia, LI Hong-yan, WANG Chuan-qing. Molecular Identification and Genetic Evolution Analysis of Haemophilus parasuis 16S rRNA Gene in Local Pig Origin[J]. , 2013, 40(11): 157-160.

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Molecular Identification and Genetic Evolution Analysis of Haemophilus parasuis 16S rRNA Gene in Local Pig Origin

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