›› 2013, Vol. 40 ›› Issue (10): 228-230.

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Isolation and Identification of Aeromonas hydrophila from Infected Tilapia in Early Spring

ZUO Yue1, YI Yi1, XIA Jie1, LUO Fu-guang2, YANG Jun2,3, LI Fang-fang1, WU Shi-hua1   

  1. 1. College of Biological and Chemical Engineering, Guangxi University of Technology, Liuzhou 545006, China;
    2. Liuzhou Fishery Technical Extension Station, Liuzhou 545006, China;
    3. Liuzhou Comprehensive Experiment Station of the National Tilapia Industry Technology System, Liuzhou 545006, China
  • Received:2013-03-27 Online:2013-10-20 Published:2013-12-19

Abstract: In early spring 2012, a disease outbreak occurred at a tilapia farm in Liuzhou of Guangxi province. A gram-negative bacillus was isolated from liver,kidney and spleen of infected tilapia. The isolate was identified as Aeromonas hydrophila by biochemical assays and 16S rDNA sequencing. Healthy tilapia injected with 1×109 CFU of the isolate reached a mortality of 100% in just 24 h. Drug sensitivity experiment showed that the isolate was only resistant to ampicillin and sensitive to 15 types of antibiotics such as aztreonam,ofloxacin,etc. This study provided a basis for the prevention and control of tilapia diseases in early spring.

Key words: tilapia; Aeromonas hydrophila; strong virulence; early spring

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