Abstract: To establish a rapid duck Tembusu virus(DTMUV)pathogen detection methods, based on DTMUV gene sequence in GenBank, we synthesized one pair of primers, established a RT-PCR for DTMUV detection. The method was used for detecting duck hepatitis virus (DHV-Ⅰ), Newcastle disease virus(NDV), egg drop syndrome virus(EDSV), duck enteritis virus (DEV), all of the PCR results were negative, the sensitivity was 1 ng RNA, the established RT-PCR method had good specificity, sensitivity, reproducibility, it could detect very low levels of DTMUV quickly and accurately, it provided a kind of rapid, sensitive, specific and precise molecular biology detection method for pathogen detection and molecular epidemiology of material such as DTMUV.

Key words: duck Ttembusu virus; RT-PCR; diagnosis