Abstract: To establish a rapid bovine viral diarrhea virus (BVDV) pathogen detection methods, based on BVDV gene sequence in GenBank, we synthesized one pair of primers, established one step RT-PCR for BVDV detection. The method was used for infectious bovine rhinotracheitis virus (IBRV), classical swine fever virus(CSFV), bovine para influenza virus 3 (BPIV3), all of the PCR results were negative, the sensitivity was 1 ng RNA. The established one step PCR method had good specificity, sensitivity, reproducibility, it could detect very low levels of BVDV quickly and accurately, it provided a kind of rapid, sensitive, specific and precise molecular biology detection method for pathogen detection and molecular epidemiology of material such as BVDV.

Key words: bovine viral diarrhea virus (BVDV); RT-PCR; detection