禽流感H9亚型一步法RT-PCR快速检测试剂盒的研制及应用

Abstract

Abstract: In order to establish a simple, sensitive, accurate and rapid PCR detection method and develop a one-step RT-PCR detection kit for AIV H9 subtype,one pair of specific primers was designed and synthesized according to the conserved gene of AIV H9 subtype in GenBank, and then PCR method of rapid detection of AIV H9 subtype was formed, and through optimizing the reaction conditions, a rapid detection kit was assembled, and lots of samples from suspected AIV H9 subtype were to be detected. The results showed that AIV H9 subtype one-step RT-PCR detection method was successfully established, and then developed a kit, in addition, the rate of detection to samples suspected AIV H9 subtype was up to 98%, the detection sensitivity was 10^1.5 ELD₅₀, and the stability was good, in the cryopreservation case, it could survive for at least 12 months. Moreover, the operation was easy, fast, economic, effective. Detection of clinical samples confirmed that the kit was simple, rapid, high sensitivity, specificity, repeatability and stability, it was worthy to be promoted and applied.

Key words: AIV; H9 RT-PCR; kit

CLC Number:

S854.4

CHENG Fu-liang, LIANG Jin, FAN Qun-ping, CHEN Tian-tian, SU Zhi-rui, NIE Zhao-jing, GU Wei. Development and Application of Rapid RT-PCR Detection Kit for AIV H9 Subtype[J]. , 2013, 40(1): 50-53.

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Development and Application of Rapid RT-PCR Detection Kit for AIV H9 Subtype

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