Abstract: 【Objective】 The aim of the experiment was to prepare a nanogel for topical delivery of kitasamycin for skin wound infections.【Method】 Chitosan/gelatin/poly(vinyl alcohol) nanogel were developed by electrostatic interaction between the gel materials under the action of Schiff base using genipin as cross-linking agent and loaded with the drug kitasamycin.The encapsulation rate,drug loading,swelling properties,nanoparticle size,Zeta potential,microstructure scanning,and other characterisation structures and biosafety were tested.The in vitro release effect and wound healing activity of nanogel were evaluated.【Result】 In the present study,the kitasamycin nanogel was prepared in the composition of 0.25 g of kitasamycin,0.4 g of chitosan,0.3 g of gelatin,0.2 g of polyvinyl alcohol,and 0.2 g genipin per 25 mL of the gel,and the remaining amount of deionized water,the appearance properties were light blue nanogel,without precipitation and drug precipitation,the swelling degree was high,the drug loading was 13.6%,and the encapsulation rate was 83.1%.Scanning electron microscopy showed that the nanogel formed a three-dimensional mesh structure with dense and uniformly distributed pores.The average particle size was 28.56 nm,Zeta potential was 16.33 mV,with good biological safety.The nanogel could release the drug in vitro for 48 h,which was significantly longer than that of the kitasamycin solution.In addition,the nanogel could reduce the wound size of mice by 96%,which was helpful for wound healing.【Conclusion】 In this study,kitasamycin nanogels were successfully prepared,and their potential ability to control drug release and promote skin wound healing was demonstrated by in vitro and in vivo evaluations.

Key words: kitasamycin; nanogel; controlled drug release; wound healing