贵州地方猪品种毛色与KIT基因拷贝数之间的关联分析

doi:10.16431/j.cnki.1671-7236.2015.03.026

Abstract

Abstract: To investigate the association of various coat color phenotypes and copy number variation (CNV) of KIT gene in pig,four indigenous pig breeds originated from Guizhou province were chosen as samples using quantitative Real-time PCR (qPCR) method,which included Qianbei black,Kele,Nuogu and Xiang pig breeds,taking Yorkshire and Rongchang pig as controls.The results showed that the dominant status of KIT CNV were loss in four Guizhou indigenous pig breeds with black/brown coat color,the KIT CNV status in pigs with black and white patch were all lose,while the increasing and normal individuals with brown and white patch were half.The main CNV of KIT gene was gain in Yorkshire pig.However,the normal status was majority in Rongchang pig breed.It confirmed that the duplication of KIT gene was the critical reason for the white coat in Yorkshire pig,but it might be not the case in Rongchang pig breed.In the four Guizhou indigenous pig breeds with black/brown coat,it might be associated with the loss of KIT gene copy.It was interested that the copy numbers of KIT gene in Kele and Xiang pig with white patched coat were detected to lose one or two copies.It suggested that the white patched coat might be unrelated with the copy number changes of KIT gene with another special mechanism in Guizhou indigenous pig breeds.

Key words: Guizhou indigenous pig breeds; Rongchang pig; KIT gene; CNV

CLC Number:

S813.3

HE Shu-fang, RAN Xue-qin, WANG Jia-fu. The Association Analysis of Copy Number of KIT Gene with Coat Color Trait in Guizhou Indigenous Pig Breeds[J]. , 2015, 42(3): 676-684.

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The Association Analysis of Copy Number of KIT Gene with Coat Color Trait in Guizhou Indigenous Pig Breeds

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