›› 2013, Vol. ›› Issue (5): 44-49.

Previous Articles     Next Articles

Expression of miR-193b and its Target Gene Kit in Different Colors of Alpaca Skin

ZHANG Dan-li1, TIAN Xue1, SONG Yun-fei2, MU Ren3, DONG Chang-sheng1   

  1. 1. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China;
    2. Beijing Liuhe Huada Gene Science and Technology Limited Company, Lanzhou 730000, China;
    3. Commonwealth Scientific and Industrial Research Organisation (CSIRO) Animal, Food and Health Sciences, Australia 2350
  • Received:2012-10-15 Online:2013-05-20 Published:2013-05-27

Abstract: This experiment was conducted to explore the expression of miR-193b and Kit in different colors of alpaca skin. The tissues were obtained from adult alpaca skins of white and brown hair colors. The comparative expression quantity of miR-193b and Kit in different hair colors was analyzed by real time quantitative PCR. The results of QRT-PCR showed that the comparative expression level of miR-193b in brown alpacas was 1.741346 times than that in white ones, and the comparative expression level of Kit in white alpacas was 4.6029 times than that in brown ones. The findings showed that miR-193b and its target gene Kit might involved in the regulation of hair color formation and miR-193b might regulate the formation of coat color by downregulating the expression of Kit.

Key words: miR-193b; Kit; hair color; QRT-PCR; alpaca

CLC Number: