鹿茸组织中内参基因的筛选和验证

doi:10.16431/j.cnki.1671-7236.2015.04.017

Abstract

Abstract: The aim of this study was to identify the most stable gene to be used as reference genes for qRT-PCR analysis in Sika deer antler tissues. The expression patterns of 6 housekeeping genes were analyzed, including glyceraldehyde-3-phosphate dehydrogenase (GAPDH), beta-2-microglobulin (B2M), NADH dehydrogenase (NADH), 60S ribosomal protein L40 (RPL40), glutathione peroxidase 7 (GPx) and beta actin (ACTB), in Sika deer antler at different stages (10, 20, 40 and 60 d). The stability of housekeeping gene expression was analyzed with use of 2 software packages, including geNorm and NormFinder. The results showed that GAPDH, ACTB, RPL40 were suitable reference genes for efficient normalization of qRT-PCR data, whereas NADH and GPx were not suitable for analysis of Sika deer antler. These findings were confirmed by comparative profiling of 5 antler genes associated with antler rapid growth (ANXA5, HSP27, PRD2, CRABP1 and LGALS1), while it was observed that the 5 genes were significanly expressed in antler aged 10 d. These results will provide a necessary basis for the further research on rapid growth of antler and ossification genes.

Key words: qRT-PCR; velvet antlers; reference genes; geNorm; NormFinder

CLC Number:

S825

ZHANG Ran-ran, LIU Hua-miao, XING Xiu-mei, HU Da-yong. Selection and Validation of Reference Genes in Velvet Antlers Tissues[J]. , 2015, 42(4): 883-889.

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Selection and Validation of Reference Genes in Velvet Antlers Tissues

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