Abstract: To explore simple, fast and practical diagnosis method for cow brucellosis in this study, we established a microscale agglutination test using 96-well "V" plate and optimized the test conditions, the optimal antigen diluted concentration was 1:20, the optimal reaction temperature was 37℃, and the optimal reaction time was 24 h. To verify the veracity microscale agglutination test, we retested 120 rose-bengal plate agglutination test (RBT) positive cow serum samples by microscale agglutination test and tube agglutination test, the coincidence rate of microscale agglutination test and tube agglutination test to RBT were 83.3% and 80.0%, respectively. These results showed that the microscale agglutination test could be used as the alternative method of tube agglutination test for cow brucellosis diagnosis and quarantine.

Key words: brucellosis; cow; microscale agglutination test; diagnosis