›› 2013, Vol. ›› Issue (5): 129-133.

Previous Articles     Next Articles

Study on Acute Toxicity Test in Mice and Sub-acute Toxicity Test in Rats of "Runingsan"

WANG Guo-qing, WANG Xu-rong, ZHANG Kai, ZHANG Jing-yan, WANG Xue-zhi, MENG Jia-ren, CHANG Rui-xiang, YANG Zhi-qiang, LI Jian-xi   

  1. Key Laboratory of New Animal Drug Project of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture, Engineering and Technology Research Center of TCVM of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Science of CAAS, Lanzhou 730050, China
  • Received:2013-01-23 Online:2013-05-20 Published:2013-05-27

Abstract: To investigate the safety of "Runingsan" for treatment and prevention of the mastitis in dairy cow, the acute and sub-acute toxicity was tested in mice and rat. "Runingsan" was extracted with water and concentrated 1.0 g/mL solution. In acute toxicity test, 50 mice were randomly divided into 5 groups, the animals were administered drug by intragastric infusion at single dose of 5000, 7500, 10000, 15000 mg/(kg·BW) respectively. Poisoning typical symptom and death cases were observedand the median lethal dose (LD50) were calculated. In order to estimate the maximum dosage, 40 mice were randomly divided into the treatment group and control group group, "Runingsan" was administered intragastrically at a maximum concentration (1.0 g/mL) and maximum capacity (0.04 mL/g) with single dose in the treatment group, 0.9% saline was used in control group. After being administered, the mice were observed for 7 days. In sub-acute toxicity test, 80 rats were randomly divided into 3 drug-treated groups (high, middle and low dose) and one control group, drug was administered orally at dose of 3000, 1500 and 750 mg/(kg·d) respectively. In control group, rats were administered 0.9% NaCl. Body weight gain, routine blood test indexes, blood biochemical parameters, organ coefficient and histopathology were detected. The results showed there was not any poisoning and death cases in each group, there was no LD50 in this herbal compound. "Runingsan" maximum oral dosage was over 40.0 g/(kg·BW) in mice. These data indicated that the drug was actually non-toxin. There were not significantly different in body weight gain, routine blood test, blood biochemical indexes and organ coefficient between 3 drug-treated groups and control group. The histopathological result was not any lesion in rat real organs of every group. All of these results suggested that "Runingsan" was safety for the dairy cow, which were in mastitis under the recommended dosage and course of the treatment in clinic.

Key words: dairy cow; sub-clinical mastitis; traditional Chinese veterinary medicine; toxicity test

CLC Number: