鸡毒支原体LAMP可视化检测方法的建立

Abstract

Abstract: A loop-mediated isothermal amplification (LAMP) assay was developed for detection of mycoplasma gallisepticum(MG). According to the sequences of MG in GenBank, six primers were designed, and the reaction conditions were optimized. The results showed that the detection limit of this LAMP method was 10 fg/μL, which was 10²-fold higher than the routine PCR. There was no cross-reactivity with the other pathogen of chicken. The amplification could be finished by water bath within 1 h, and the presence of MG could be detected and visualized by naked eyes. These results suggested that this LAMP assay was a simple and specific method for rapid detection of MG of clinical samples.

Key words: mycoplasma gallisepticum; LAMP; visual detection

CLC Number:

LUO Si-si, XIE Zhi-xun, DENG Xian-wen, XIE Zhi-qin, PANG Yao-shan, XIE Li-ji, LIU Jia-bo, FAN Qing, PENG Yi. Development of a Loop-Mediated Isothermal Amplification Assay for Visual Detection of Mycoplasma Gallisepticum[J]. .

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Development of a Loop-Mediated Isothermal Amplification Assay for Visual Detection of Mycoplasma Gallisepticum

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