食品动物源产CTX-M-14大肠杆菌传播分子机制的演变

Abstract

Abstract: Sixteen CTX-M-14 producing Escherichia coli isolates from food animal recovered from 2002 to 2009 were subjected to detection of extended spectrum beta-lactamases (ESBLs) encoding genes,plasmid mediated quinolone resistance determinants,and other relevant resistant genes (rmtB and floR); strains typing using PFGE and phylogrouping; bla_CTX-M-14 plasmid replicon typing,transferring of bla_CTX-M-14by conjugation,and detection of context of bla_CTX-M-14 gene. Majority of 16 isolates were clonally unrelated by PFGE,suggesting horizontally transfer of bla_CTX-M-14 among food animals,and corresponded to phylogenetic groups A (50%),D (21.5%) and B1 (28.5%). Plasmids containing bla_CTX-M-14a were belonging to IncK (n=3),IncHI2 (n=1),IncHI1 and IncN (n=2),IncFIB and IncF (n=1),IncF (n=5),or IncI1 (n=1),with insertion sequence of ISEcp1 upstream bla_CTX-M-14a,and apparently increased in numbers of replicon and diversity from 2002 to 2009. The increasing dissemination of bla_CTX-M-14 among different food-producing animals is mostly mediated by IncK,IncF,IncHI2,IncI1 plasmids carried by commensal E.coli. Some of CTX-M-14-producing E.coli harbored simultaneously bla_CTX-M-79or bla_TEM-135, as well other relevant resistant genes,such as oqxA, floR, aac(6')-1b-cr or rmtB. Bla_CTX-M-14could be transferred separately in isolates of 2002,while co-transferred with other relevant resistant genes on the same plasmid via conjugation or transformation,suggesting co-selection and spread of CTX-M-14 producing isolates when use of any of the relevant antibiotics in animal farms.

Key words: CTX-M-14; molecular mechanism of transmission; replicon typing; horizontal transmission

CLC Number:

S852.61

ZHEN Pan-pan, TANG Dian, REN Yan-na, GUO Yu-fang, WANG Li-hua, QIU Zong-ping, JIANG Hong-xia. Evolutionally Dissemination of CTX-M-14 Producing Escherichia coli among Food-producing Animals[J]. .

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Evolutionally Dissemination of CTX-M-14 Producing Escherichia coli among Food-producing Animals

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